Low IGF-I in synovial fluid and serum in patients with aseptic prosthesis loosening

• Published in: Acta orthopaedica Vol. 76; no. 3; pp. 320 - 325
• Main Authors: Andersson, Martin K, Stark, André, Anissian, Lucas, Mohan, Subburaman, Tsai, Jon A
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 2005; Taylor & Francis
• Subjects: Aged; Arthroplasty, Replacement, Hip - adverse effects; Cell Proliferation; Cells, Cultured; Humans; Insulin-Like Growth Factor Binding Proteins - analysis; Insulin-Like Growth Factor Binding Proteins - blood; Insulin-Like Growth Factor Binding Proteins - physiology; Insulin-Like Growth Factor I - analysis; Insulin-Like Growth Factor I - physiology; Insulin-Like Growth Factor II - analysis; Medicin och hälsovetenskap; Middle Aged; Osteoarthritis, Hip - blood; Osteoarthritis, Hip - metabolism; Osteoarthritis, Hip - surgery; Osteoblasts - physiology; Prosthesis Failure; Reoperation; Synovial Fluid - chemistry
• ISSN: 1745-3674; 1745-3682
• DOI: 10.1080/00016470510030760

Background We have previously shown that proliferation in primary cultures of human osteoblast-like cells is lower after exposure to synovial fluid from patients with aseptic prosthesis loosening than after exposure to synovial fluid from patients with osteoarthrosis. Materials and methods Insulin-like growth factors (IGF) I and II and IGF binding proteins (IGFBP) 3-6, were measured with radioimmunoassy in synovial fluid and in serum from patients with aseptic prosthesis loosening or osteoarthrosis. Proliferation in osteoblast-like MG-63 cells was studied with the CyQUANT assay. Results IGF-I and IGFBP-4 concentrations were lower whereas the concentration of IGFBP-6 was higher in synovial fluids from patients with prosthesis loosening than in synovial fluid from patients with osteoarthrosis. IGF-I concentrations in serum from patients with prosthesis loosening were also lower than in the osteoarthrosis group, and were even below the normal range in most cases (72%). Synovial fluid from patients with aseptic loosening had a weaker stimulatory effect on MG63 osteoblast-like cell proliferation than synovial fluid from patients with osteoarthrosis, but there was no difference between the two groups when a human IGF-I antibody was added. Interpretation Low levels of IGF-I in synovial fluid possibly result from low serum levelsand may be a mechanism leading to aseptic prosthesis loosening.