Sex-Specific Modulation of Cell Proliferation by Socially Relevant Stimuli in the Adult Green Treefrog Brain (Hyla cinerea)

• Published in: Brain, behavior and evolution Vol. 74; no. 2; pp. 143 - 154
• Main Authors: Almli, Lynn M., Wilczynski, Walter
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2009
• Subjects: Acoustic Stimulation; Amphibians; Analysis of Variance; Androgens - blood; Animals; Anura; Anura - physiology; Brain - metabolism; Brain - physiology; Breeding seasons; Bromodeoxyuridine - metabolism; Cell Count; Cell Proliferation; Estrogens; Estrogens - blood; Female; Females; Hormones; Hyla cinerea; Immunoenzyme Techniques; Immunohistochemistry; Male; Original Paper; Sex Characteristics; Sexual behavior; Sexual Behavior, Animal - physiology; Social Environment; Steroid hormones; Vocalization, Animal - physiology; Cell proliferation; Acoustic communication; Social behavior; Neurogenesis; Amphibian; BrdU
• DOI: 10.1159/000235963
• ISSN: 0006-8977

Social experience plays an important role in regulating the neural, physiological and hormonal changes that accompany the expression of reproductive behavior in vertebrates. This suite of functions is sexually dimorphic, with different neural control areas preeminent in males and females. In anuran amphibians, social experience comes in the form of acoustic communication, which is central to their reproductive behavior. We sought to determine whether acoustic cues regulate cell proliferation in the brain of adult green treefrogs (Hyla cinerea). Our results show that both male and female treefrogs that heard a conspecific chorus during the breeding season exhibited increased brain cell proliferation compared to animals that heard random tones. Increased cell proliferation, as assessed by the number of 5-bromo-2′-deoxyuridine-immunoreactive (BrdU+) cells, were found near the ventricles of acoustically sensitive brain regions such as the preoptic area (POA) and the infundibular hypothalamus (IF). Sex differences emerged in the location of this socially modulated cell proliferation: increases occurred primarily in the male POA and the female IF. In addition, gonadal steroid hormones might have played a role in the social modulation of cell proliferation: by statistically control- ling for hormone level, we revealed that androgens might influence socially induced increases in BrdU+ cells in the male POA, but estrogen did not contribute to socially induced increases in the female IF. These results indicate that the reception of social cues increases cell proliferation in brain regions mediating sexual behavior and endocrine regulation, and moreover that social modulation of cell proliferation occurs in a sexually differentiated fashion.