Rapid detection of the red fire ant Solenopsis invicta (Hymenoptera: Formicidae) by loop-mediated isothermal amplification

• Published in: Applied Entomology and Zoology Vol. 54; no. 3; pp. 319 - 322
• Main Authors: Nakajima, Nobuyoshi, Sakamoto, Yoshiko, Goka, Koichi
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Science and Business Media LLC 01.08.2019; Springer Japan; Springer Nature B.V
• Subjects: Agriculture; Applied Ecology; Biomedical and Life Sciences; Cytochrome-c oxidase; DNA; Entomology; Environmental Management; Introduced species; Invasive species; Japan; Life Sciences; loop-mediated isothermal amplification; Mitochondria; Nucleotide sequence; nucleotide sequences; Plant Pathology; Primers; rapid methods; Solenopsis invicta; Technical Note; Turbidity; Zoology; Japan; Loop-mediated isothermal amplification; Invasive alien species

We developed a loop-mediated isothermal amplification (LAMP) assay to detect the red imported fire ant, Solenopsis invicta Buren, an invasive species in Japan. We designed species-specific LAMP primers on the basis of the nucleotide sequence of the S. invicta mitochondrial cytochrome c oxidase subun...