Direct cytosolic delivery of cargoes in vivo by a chimera consisting of D- and L-arginine residues

• Published in: Journal of controlled release Vol. 162; no. 2; pp. 286 - 294
• Main Authors: Ma, Yan, Gong, Cheng, Ma, Yilong, Fan, Fengkai, Luo, Meijie, Yang, Fei, Zhang, Yu-Hui
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 10.09.2012; Elsevier
• Subjects: Animals; Arginine - chemistry; bioavailability; Biological and medical sciences; Biological Transport; Cell Membrane - metabolism; Cell Survival - drug effects; Cell-penetrating peptide; Cell-Penetrating Peptides - administration & dosage; Cell-Penetrating Peptides - chemistry; Cell-Penetrating Peptides - pharmacokinetics; chimerism; cultured cells; cytosol; Cytosol - metabolism; Cytosolic delivery; Drug Carriers - administration & dosage; Drug Carriers - chemistry; Drug Carriers - pharmacokinetics; drugs; endocytosis; Fluorescent Dyes - administration & dosage; Fluorescent Dyes - pharmacokinetics; General pharmacology; guidelines; HEK293 Cells; HeLa Cells; Heterogeneous; Humans; hybrids; intravenous injection; Medical sciences; membrane potential; Mice; Oligoarginine; Oligopeptides - administration & dosage; Oligopeptides - chemistry; Oligopeptides - pharmacokinetics; peptides; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Rhodamines - administration & dosage; Rhodamines - pharmacokinetics; Systemic toxicity; therapeutics; toxicity; Heterogeneous; Oligoarginine; Systemic toxicity; Cytosolic delivery; Cell-penetrating peptide; Pharmaceutical technology; Cell penetrating peptide; Toxicity; Cytosol; In vivo; Arginine; Residue; Aminoacid; Chimera

The ability of cell-penetrating peptides (CPPs) to deliver a range of membrane-impermeable molecules into living cells makes them attractive potential vehicles for therapeutics. However, in vivo, the efficiency of CPP delivery to the cytosol remains unsatisfactory owing to endosomal entrapment and/o...