Quantification of the Latent HIV-1 Reservoir Using Ultra Deep Sequencing and Primer ID in a Viral Outgrowth Assay

• Published in: Journal of acquired immune deficiency syndromes (1999) Vol. 74; no. 2; p. 221
• Main Authors: Lee, Sook-Kyung, Zhou, Shuntai, Baldoni, Pedro L, Spielvogel, Ean, Archin, Nancie M, Hudgens, Michael G, Margolis, David M, Swanstrom, Ronald
• Format: Journal Article
• Language: English
• Published: United States 01.02.2017
• Subjects: Anti-Retroviral Agents - therapeutic use; CD4-Positive T-Lymphocytes - virology; DNA Primers - genetics; Genetic Variation; Genotype; High-Throughput Nucleotide Sequencing; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - classification; HIV-1 - genetics; HIV-1 - isolation & purification; Humans; Phylogeny

In this study, we measured the latent HIV-1 reservoir harboring replication-competent HIV-1 in resting CD4 T cells in participants on highly active antiretroviral therapy, quantitating the frequency of latent infection through the use of a Primer ID-based Ultra Deep Sequencing Assay (UDSA), in compa...