Sequence Elements Distal to the Ligand Binding Pocket Modulate the Efficiency of a Synthetic Riboswitch

• Published in: Chembiochem : a European journal of chemical biology Vol. 15; no. 11; pp. 1627 - 1637
• Main Authors: Weigand, Julia E., Gottstein-Schmidtke, Sina R., Demolli, Shemsi, Groher, Florian, Duchardt-Ferner, Elke, Wöhnert, Jens, Suess, Beatrix
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 21.07.2014; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: aptamers; Aptamers, Nucleotide - genetics; Aptamers, Nucleotide - metabolism; Binding Sites; Biological activity; Efficiency; Gene expression; Ligands; Models, Molecular; Mutation; Neomycin - metabolism; NMR spectroscopy; Riboswitch - genetics; riboswitches; RNA structures; synthetic biology; synthetic biology; RNA structures; riboswitches; NMR spectroscopy; aptamers; gene expression

Synthetic riboswitches can serve as sophisticated genetic control devices in synthetic biology, regulating gene expression through direct RNA–ligand interactions. We analyzed a synthetic neomycin riboswitch, which folds into a stem loop structure with an internal loop important for ligand binding an...