Compaction of Single-Molecule Megabase-Long Chromatin under the Influence of Macromolecular Crowding

• Published in: Biophysical journal Vol. 114; no. 10; pp. 2326 - 2335
• Main Authors: Zinchenko, Anatoly, Berezhnoy, Nikolay V., Chen, Qinming, Nordenskiöld, Lars
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.05.2018; The Biophysical Society
• Subjects: Bacteriophage T4; Chromatin - drug effects; Chromatin - metabolism; DNA, Viral - metabolism; Histones - metabolism; Humans; Magnesium - pharmacology; Nucleic Acids and Genome Biophysics; Nucleosomes - drug effects; Nucleosomes - metabolism; Polyethylene Glycols - pharmacology; Sodium - pharmacology

The megabase-sized length of chromatin is highly relevant to the state of chromatin in vivo, where it is subject to a highly crowded environment and is organized in topologically associating domains of similar dimension. We developed an in vitro experimental chromatin model system reconstituted from...