Enhancement of Brassica napus Tolerance to High Saline Conditions by Seed Priming

• Published in: Plants (Basel) Vol. 10; no. 2; p. 403
• Main Authors: Stassinos, Panaiotis M., Rossi, Massimiliano, Borromeo, Ilaria, Capo, Concetta, Beninati, Simone, Forni, Cinzia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.02.2021; MDPI
• Subjects: Abiotic stress; antioxidant activities; Antioxidants; Brassica; Brassica napus; Crop production; Cultivars; Gene expression; Genotypes; Membranes; Oxidative stress; Photosynthesis; Photosynthetic pigments; Physiological effects; Physiological responses; Physiology; Pigments; Plant growth; Polyamines; Priming; Productivity; Proline; Rape plants; Rapeseed; Saline soils; Salinity; Salinity effects; Salinization; Salt; salt stress; Salts; seed priming; Seed treatments; Seeds; Sodium chloride; Soil salinity; soil salinization; Spermidine; Spermine; Stress response; Transcription factors; proline; spermidine; antioxidant activities; seed priming; salt stress; rapeseed
• DOI: 10.3390/plants10020403
• ISSN: 2223-7747

Plants grown in saline soils undergo osmotic and oxidative stresses, affecting growth and photosynthesis and, consequently, the yield. Therefore, the increase in soil salinity is a major threat to crop productivity worldwide. Plant’s tolerance can be ameliorated by applying simple methods that induce them to adopt morphological and physiological adjustments to counteract stress. In this work, we evaluated the effects of seed priming on salt stress response in three cultivars of rapeseed (Brassica napus L.) that had different tolerance levels. Seed chemical priming was performed with 2.5 mM spermine (SPM), 5 mM spermidine (SPD), 40 mM NaCl and 2.5 mM Ca (NO3)2. Primed and not primed seeds were sown on saline and not saline (controls) media, and morphological and physiological parameters were determined. Since SPD treatment was effective in reducing salinity negative effects on growth, membrane integrity and photosynthetic pigments, we selected this priming to further investigate plant salt stress response. The positive effects of this seed treatment on growth and physiological responses were evident when primed plants were compared to not primed ones, grown under the same saline conditions. SPD priming ameliorated the tolerance towards saline stress, in a genotype-independent manner, by increasing photosynthetic pigments, proline amounts and antioxidant responses in all cultivars exposed to salt. These results may open new perspectives for crop productivity in the struggle against soil salinization.