Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3663
• Main Authors: Franco, Chiara, Ghirardello, Anna, Bertazza, Loris, Gasparotto, Michela, Zanatta, Elisabetta, Iaccarino, Luca, Valadi, Hadi, Doria, Andrea, Gatto, Mariele
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.02.2023; MDPI
• Subjects: Autoimmune diseases; Biochemistry & Molecular Biology; Biomarkers; Blood; Cell culture; Chemistry; Chromatography; Chromatography, Gel - methods; Disease; Ethylenediaminetetraacetic acid; Extracellular vesicles; Extracellular Vesicles - chemistry; Flow cytometry; Humans; idiopathic inflammatory myopathies; Lipoproteins - metabolism; Membrane lipids; Microscopy, Electron, Transmission; Molecular Biology; Molekylärbiologi; Morphology; Nanoparticles; Plasma; polymyositis; Proteins; size-exclusion chromatography; ultrafiltration; Ultrafiltration - methods; idiopathic inflammatory myopathies; extracellular vesicles; ultrafiltration; size-exclusion chromatography
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043663

There is still a need for an efficient method for the isolation of extracellular vesicles (EVs) from human blood that provides a reliable yield with acceptable purity. Blood is a source of circulating EVs, but soluble proteins and lipoproteins hamper their concentration, isolation, and detection. Th...