Transcriptional regulator-mediated activation of adaptation genes triggers CRISPR de novo spacer acquisition

• Published in: Nucleic acids research Vol. 43; no. 2; pp. 1044 - 1055
• Main Authors: Liu, Tao, Li, Yingjun, Wang, Xiaodi, Ye, Qing, Li, Huan, Liang, Yunxiang, She, Qunxin, Peng, Nan
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 30.01.2015
• Subjects: Binding Sites; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Proteins - genetics; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems; DNA, Archaeal - chemistry; DNA, Archaeal - metabolism; Molecular Biology; Promoter Regions, Genetic; Sulfolobus - genetics; Sulfolobus - metabolism; Trans-Activators - metabolism; Transcriptional Activation

Acquisition of de novo spacer sequences confers CRISPR-Cas with a memory to defend against invading genetic elements. However, the mechanism of regulation of CRISPR spacer acquisition remains unknown. Here we examine the transcriptional regulation of the conserved spacer acquisition genes in Type I-...