Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells

• Published in: Nature methods Vol. 10; no. 12; pp. 1232 - 1238
• Main Authors: Kiyonaka, Shigeki, Kajimoto, Taketoshi, Sakaguchi, Reiko, Shinmi, Daisuke, Omatsu-Kanbe, Mariko, Matsuura, Hiroshi, Imamura, Hiromi, Yoshizaki, Takenao, Hamachi, Itaru, Morii, Takashi, Mori, Yasuo
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.12.2013; Nature Publishing Group
• Subjects: 631/1647/1888/2249; 631/443/319; 631/80/2373; Adenoviridae - genetics; Adipocytes, Brown - cytology; Bacterial Proteins - chemistry; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical Engineering/Biotechnology; Biosensors; Birds; Body temperature; Cells; DNA, Complementary - metabolism; Electrochemistry; Escherichia coli - metabolism; Fluorescence; Genetic aspects; Genetics; Green Fluorescent Proteins - chemistry; HeLa Cells; Heterogeneity; Hot Temperature; Humans; Life Sciences; Luminescent Proteins - metabolism; Mammals; Microscopy, Fluorescence - methods; Mitochondria - metabolism; Mitochondrial DNA; Molecular biology; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - metabolism; Physiological aspects; Protein Conformation; Proteomics; Regulation; Salmonella; Salmonella enterica - metabolism; Sensors; Temperature; Japan

Genetically encoded sensors based on GFP enable the visualization of subcellular thermal changes noninvasively in intact cells. In mammals and birds, thermoregulation to conserve body temperature is vital to life. Multiple mechanisms of thermogeneration have been proposed, localized in different sub...