FRET Image Correlation Spectroscopy Reveals RNAPII-Independent P-TEFb Recruitment on Chromatin

• Published in: Biophysical journal Vol. 114; no. 3; pp. 522 - 533
• Main Authors: Bidaux, Gabriel, Le Nézet, Corentin, Pisfil, Mariano Gonzalez, Henry, Mélanie, Furlan, Alessandro, Bensaude, Oliver, Vandenbunder, Bernard, Héliot, Laurent
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.02.2018; Biophysical Society; The Biophysical Society
• Subjects: Biological Physics; Cells; Cells (biology); Chromatin; Clusters; Correlation analysis; DNA-directed RNA polymerase; Elongation; Energy transfer; Fluorescence; Fluorescence resonance energy transfer; Genetic engineering; Histidine; Histone H2A; Image acquisition; Image analysis; Image processing; Microscopy; Molecular interactions; Nuclei; Nucleic Acids and Genome Biophysics; Phosphorylation; Physics; Protein interaction; Proteins; Recruitment; Ribonucleic acid; RNA; RNA polymerase II; Spatial distribution; Spectroscopy; Spectrum analysis; Statistical analysis; Transcription elongation factor b

Biochemical studies have revealed that the RNA Polymerase II (RNAPII) pause release is triggered by phosphorylation of the transcription machinery by the positive transcription elongation factor b (P-TEFb). However, there are no direct report that P-TEFb and RNA polymerase II interact in single livi...