A Nonsense Mutation in Zebrafish gata1 Causes the Bloodless Phenotype in vlad tepes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 99; no. 8; pp. 5454 - 5459
• Main Authors: Lyons, Susan E., Lawson, Nathan D., Lei, Lin, Bennett, Paul E., Weinstein, Brant M., Liu, P. Paul
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 16.04.2002; National Acad Sciences
• Subjects: 3T3 Cells; Alleles; Animals; Biological Sciences; Blood cells; Centromere; Codon, Nonsense; DNA; DNA - metabolism; DNA, Complementary - metabolism; DNA-Binding Proteins - metabolism; Embryos; Erythroid-Specific DNA-Binding Factors; Fish; GATA1 Transcription Factor; Genes; Genetic Linkage; Genetic mutation; Genotype; Homozygote; Humans; In Situ Hybridization; Mice; Mutation; Nonsense mutation; Phenotype; Phenotypes; Polymerase Chain Reaction; Protein Binding; RNA; Stem cells; Transcription Factors - metabolism; Transcriptional Activation; Zebrafish; Zebrafish Proteins

Vlad tepes (vltm651) is one of only five "bloodless" zebrafish mutants isolated through large-scale chemical mutagenesis screening. It is characterized by a severe reduction in blood cell progenitors and few or no blood cells at the onset of circulation. We now report characterization of t...