Identification of the major photodegradant in metronidazole by LC-PDA-MS and its reveal in compendial methods

• Published in: Scientific reports Vol. 12; no. 1; pp. 11665 - 6
• Main Authors: Chen, Mei-Ling, Xu, Hong-Xia, Yuan, Wei-Feng, Zhao, Sui-Hong, Li, Xue, Zhu, Lan-Xin, Shen, Zong-Yong, Liu, Yu-Jing, Wang, Ming-Juan, Ma, Ang, Hoogmartens, Jos, Adams, Erwin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.07.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 639/638; 639/638/11/296; 692/308; 692/499; Chromatography; Humanities and Social Sciences; Impurities; Irradiation; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metronidazole; multidisciplinary; NMR; Nuclear magnetic resonance; Pharmaceuticals; Photodegradation; Science; Science (multidisciplinary); Scientific imaging; Software; Ultraviolet radiation; Vagina

Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N -(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further ve...