Identification of the major photodegradant in metronidazole by LC-PDA-MS and its reveal in compendial methods

Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N -(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further ve...

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Published inScientific reports Vol. 12; no. 1; pp. 11665 - 6
Main Authors Chen, Mei-Ling, Xu, Hong-Xia, Yuan, Wei-Feng, Zhao, Sui-Hong, Li, Xue, Zhu, Lan-Xin, Shen, Zong-Yong, Liu, Yu-Jing, Wang, Ming-Juan, Ma, Ang, Hoogmartens, Jos, Adams, Erwin
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 08.07.2022
Nature Publishing Group
Nature Portfolio
Subjects
639/638
639/638/11/296
692/308
692/499
Chromatography
Humanities and Social Sciences
Impurities
Irradiation
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metronidazole
multidisciplinary
NMR
Nuclear magnetic resonance
Pharmaceuticals
Photodegradation
Science
Science (multidisciplinary)
Scientific imaging
Software
Ultraviolet radiation
Vagina
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Abstract Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N -(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance ( 1 H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.
AbstractList Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N -(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance ( 1 H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.
Abstract Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N-(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance (1H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.
Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N-(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance (1H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N-(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance (1H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.
Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N-(2-hydroxyethyl)-5-methyl-l,2,4-oxadiazole-3-carboxamide. This is revealed here by liquid chromatography with tandem photo diode array detection and mass spectrometry (LC-PDA-MS) and further verified by comparison with the corresponding reference substance and proton nuclear magnetic resonance (1H-NMR). However, in current compendial tests for related substances/organic impurities of metronidazole, the above photolytic degradant could not be detected. Thus, when photodegradation of metronidazole occurs, it could not be demonstrated. In our study, an improved LC method was developed and validated, which includes a detection at a wavelength of 230 nm and optimization of mobile phase composition thereby a better separation was obtained.
ArticleNumber 11665
Author Ma, Ang
Yuan, Wei-Feng
Li, Xue
Wang, Ming-Juan
Xu, Hong-Xia
Zhu, Lan-Xin
Zhao, Sui-Hong
Adams, Erwin
Shen, Zong-Yong
Chen, Mei-Ling
Liu, Yu-Jing
Hoogmartens, Jos
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CitedBy_id crossref_primary_10_1016_j_surfin_2025_105917
crossref_primary_10_1007_s44371_025_00109_y
crossref_primary_10_3390_pr11010170
Cites_doi 10.1039/C9RA10871H
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10.1039/C7RA06341E
10.1002/jps.2600800305
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10.3390/ijms23042187
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10.1371/journal.pone.0214598
10.1111/j.1751-1097.1988.tb08834.x
10.2147/DDDT.S239458
10.1039/C9RA09115G
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Snippet Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of N...
Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of...
Abstract Metronidazole in aqueous solution is sensitive to light and UV irradiation, leading to the formation of...
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SubjectTerms 639/638
639/638/11/296
692/308
692/499
Chromatography
Humanities and Social Sciences
Impurities
Irradiation
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metronidazole
multidisciplinary
NMR
Nuclear magnetic resonance
Pharmaceuticals
Photodegradation
Science
Science (multidisciplinary)
Scientific imaging
Software
Ultraviolet radiation
Vagina
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Title Identification of the major photodegradant in metronidazole by LC-PDA-MS and its reveal in compendial methods
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Volume 12
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