Collective forces of tumor spheroids in three-dimensional biopolymer networks
We describe a method for quantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-dimensional collagen networks. While three-dimensional traction force microscopy for single cells in a nonlinear matrix is computationally complex due to the variable cell sh...
Saved in:
Published in | eLife Vol. 9 |
---|---|
Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
eLife Sciences Publications Ltd
30.04.2020
eLife Sciences Publications, Ltd |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | We describe a method for quantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-dimensional collagen networks. While three-dimensional traction force microscopy for single cells in a nonlinear matrix is computationally complex due to the variable cell shape, here we exploit the spherical symmetry of tumor spheroids to derive a scale-invariant relationship between spheroid contractility and the surrounding matrix deformations. This relationship allows us to directly translate the magnitude of matrix deformations to the total contractility of arbitrarily sized spheroids. We show that our method is accurate up to strains of 50% and remains valid even for irregularly shaped tissue samples when considering only the deformations in the far field. Finally, we demonstrate that collective forces of tumor spheroids reflect the contractility of individual cells for up to 1 hr after seeding, while collective forces on longer timescales are guided by mechanical feedback from the extracellular matrix. |
---|---|
AbstractList | We describe a method for quantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-dimensional collagen networks. While three-dimensional traction force microscopy for single cells in a nonlinear matrix is computationally complex due to the variable cell shape, here we exploit the spherical symmetry of tumor spheroids to derive a scale-invariant relationship between spheroid contractility and the surrounding matrix deformations. This relationship allows us to directly translate the magnitude of matrix deformations to the total contractility of arbitrarily sized spheroids. We show that our method is accurate up to strains of 50% and remains valid even for irregularly shaped tissue samples when considering only the deformations in the far field. Finally, we demonstrate that collective forces of tumor spheroids reflect the contractility of individual cells for up to 1 hr after seeding, while collective forces on longer timescales are guided by mechanical feedback from the extracellular matrix. |
Author | Grummel, Nadine Strick, Reiner Gerum, Richard Strissel, Pamela L Beckmann, Matthias W Hack, Carolin C Mark, Christoph Steinwachs, Julian O'Neill, Geraldine M Grundy, Thomas J Fabry, Ben Böhringer, David Eckstein, Markus |
Author_xml | – sequence: 1 givenname: Christoph orcidid: 0000-0002-8612-6469 surname: Mark fullname: Mark, Christoph organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 2 givenname: Thomas J surname: Grundy fullname: Grundy, Thomas J organization: School of Medical Sciences and Children's Hospital at Westmead Clinical School, University of Sydney, Sydney, Australia – sequence: 3 givenname: Pamela L surname: Strissel fullname: Strissel, Pamela L organization: Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 4 givenname: David surname: Böhringer fullname: Böhringer, David organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 5 givenname: Nadine surname: Grummel fullname: Grummel, Nadine organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 6 givenname: Richard surname: Gerum fullname: Gerum, Richard organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 7 givenname: Julian surname: Steinwachs fullname: Steinwachs, Julian organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 8 givenname: Carolin C surname: Hack fullname: Hack, Carolin C organization: Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 9 givenname: Matthias W surname: Beckmann fullname: Beckmann, Matthias W organization: Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 10 givenname: Markus surname: Eckstein fullname: Eckstein, Markus organization: Institute of Pathology, University Hospital Erlangen, Erlangen, Germany – sequence: 11 givenname: Reiner surname: Strick fullname: Strick, Reiner organization: Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany – sequence: 12 givenname: Geraldine M surname: O'Neill fullname: O'Neill, Geraldine M organization: School of Medical Sciences and Children's Hospital at Westmead Clinical School, University of Sydney, Sydney, Australia – sequence: 13 givenname: Ben surname: Fabry fullname: Fabry, Ben organization: Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32352379$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkc1rFDEYh4NUbF178i4DXgSZms9JchFk8aOw4kXBW8gk73SzZiZrMlPpf2-6W0trLm_y5uHJS37P0cmUJkDoJcEXUgj-DjZhgAtBNKFP0BnFArdY8Z8nD_an6LyUHa5LcqWIfoZOGWWCMqnP0Nd1ihHcHK6hGVJ2UJo0NPMyptyU_RZyCr40YWrmbQZofRhhKiFNNjZ9SPsUb0bIzQTzn5R_lRfo6WBjgfO7ukI_Pn38vv7Sbr59vlx_2LROEDm3qu_dILkQlmNrHbVdZ7lw0Kt6lIrjjmniOys6DG6wDITw2BPLueeYemArdHn0-mR3Zp_DaPONSTaYQyPlK2PzHFwEw7lQWAPTQnecEKW5o5xAfXGo6uHW9f7o2i_9CN7BNGcbH0kf30xha67StZFEU6FIFby5E-T0e4EymzEUBzHaCdJSDGW6U4JLxSr6-j90l5ZcP7NSHEutqK7YCr09Ui6nUjIM98MQbG5TN4fUzSH1Sr96OP89-y9j9hfai6t8 |
CitedBy_id | crossref_primary_10_7554_eLife_73020 crossref_primary_10_1063_5_0046093 crossref_primary_10_3389_fcell_2022_809738 crossref_primary_10_1038_s41598_024_63642_3 crossref_primary_10_1038_s41377_024_01471_9 crossref_primary_10_1038_s41467_022_30995_0 crossref_primary_10_1063_5_0191765 crossref_primary_10_1021_acs_biomac_3c01022 crossref_primary_10_1038_s42254_020_0184_6 crossref_primary_10_3390_cells12091256 crossref_primary_10_1146_annurev_bioeng_103122_031130 crossref_primary_10_1039_D0SM01451F crossref_primary_10_1088_1758_5090_abe025 crossref_primary_10_1039_D3SM01425H crossref_primary_10_1186_s12935_021_01853_8 crossref_primary_10_1063_5_0188186 crossref_primary_10_1371_journal_pcbi_1010471 crossref_primary_10_3389_fcell_2023_1239749 crossref_primary_10_1016_j_matbio_2023_11_004 crossref_primary_10_1038_s41598_021_81470_7 crossref_primary_10_3389_fbioe_2022_1060895 crossref_primary_10_3390_cells12202474 crossref_primary_10_1002_adfm_202311139 crossref_primary_10_1002_advs_202104808 crossref_primary_10_1039_D1SM90194J crossref_primary_10_1186_s13578_023_01125_0 |
Cites_doi | 10.1038/s41467-017-01011-7 10.1038/srep39498 10.1038/nprot.2008.226 10.1039/B610522J 10.1073/pnas.1222787110 10.1038/nmeth.3685 10.1038/ncomms14056 10.1038/nature03521 10.1073/pnas.1722619115 10.3171/2016.6.JNS152797 10.1186/s13058-016-0688-2 10.1529/biophysj.105.061994 10.1371/journal.pone.0009808 10.1152/ajpcell.00270.2001 10.1021/acs.chemrev.8b00042 10.1002/cpcb.24 10.1016/j.actbio.2018.11.046 10.1242/dmm.004077 10.1038/nrn3765 10.1038/nmat3025 10.1038/nphys1269 10.1073/pnas.1610347113 10.1371/journal.pone.0033476 10.1038/nmeth.1531 10.1002/bit.26845 10.1016/S0006-3495(99)77386-8 10.1016/bs.mcb.2014.10.017 10.18632/oncotarget.5874 10.1371/journal.pone.0156442 10.1073/pnas.1613058113 10.1016/j.ccr.2007.01.013 10.1007/s10585-013-9565-x 10.1109/TIM.2010.2047149 10.1038/nrc1075 10.1021/acs.nanolett.7b04982 10.1103/PhysRevE.92.032728 10.1038/s41467-019-10919-1 10.1038/nrm2720 10.1115/1.4038744 10.1109/MCSE.2007.55 10.1109/TSMC.1979.4310076 10.1002/nme.2579 10.1158/1541-7786.MCR-13-0236 |
ContentType | Journal Article |
Copyright | 2020, Mark et al. 2020, Mark et al. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2020, Mark et al 2020 Mark et al |
Copyright_xml | – notice: 2020, Mark et al. – notice: 2020, Mark et al. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2020, Mark et al 2020 Mark et al |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7X7 7XB 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PIMPY PQEST PQQKQ PQUKI PRINS Q9U 7X8 5PM DOA |
DOI | 10.7554/eLife.51912 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) Medical Database Science Database Biological Science Database Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Publicly Available Content Database ProQuest Central Student ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Central Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic CrossRef MEDLINE Publicly Available Content Database |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 2050-084X |
ExternalDocumentID | oai_doaj_org_article_445809e39596411894c241e66afcfafe 10_7554_eLife_51912 32352379 |
Genre | Video-Audio Media Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NHLBI NIH HHS grantid: P01 HL120839 – fundername: Deutsche Forschungsgemeinschaft grantid: RTG 1962 – fundername: Deutsche Forschungsgemeinschaft grantid: STR 923/6-1 – fundername: Deutsche Forschungsgemeinschaft grantid: TRR 225 PROJECT 326998133 – fundername: Deutsche Forschungsgemeinschaft grantid: FA-336/11-1 – fundername: ; – fundername: ; grantid: FA-336/11-1 – fundername: ; grantid: STR 923/6-1 – fundername: ; grantid: TRR 225 project 326998133 (subproject C02) – fundername: ; grantid: TRR 225 project 326998133 (subproject B01) – fundername: ; grantid: RTG 1962 – fundername: ; grantid: HL120839 |
GroupedDBID | 3V. 53G 5VS 7X7 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAKDD ABUWG ACGFO ACGOD ACPRK ADBBV ADRAZ AENEX AFKRA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU CGR CUY CVF DIK DWQXO ECM EIF EMOBN FRP FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HMCUK HYE IAO IEA IHR INH INR ISR ITC KQ8 LK8 M1P M2P M48 M7P M~E NPM NQS OK1 PGMZT PIMPY PQQKQ PROAC PSQYO RHF RHI RNS RPM UKHRP AAYXX CITATION 7XB 8FK K9. PQEST PQUKI PRINS Q9U 7X8 5PM AFPKN |
ID | FETCH-LOGICAL-c517t-8bbcf7455a40aac2a66a45ceb80aa78406391d6a560ecfa3e55d0d1a44d402de3 |
IEDL.DBID | RPM |
ISSN | 2050-084X |
IngestDate | Tue Oct 22 15:13:00 EDT 2024 Tue Sep 17 21:13:40 EDT 2024 Sat Oct 26 05:53:08 EDT 2024 Fri Dec 06 09:21:51 EST 2024 Fri Dec 06 06:22:51 EST 2024 Sat Nov 02 12:23:13 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | collective force generation biopolymer gels multicellular aggregates cancer biology traction force microscopy human physics of living systems tumor spheroids tumoroids |
Language | English |
License | 2020, Mark et al. http://creativecommons.org/licenses/by/4.0 This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c517t-8bbcf7455a40aac2a66a45ceb80aa78406391d6a560ecfa3e55d0d1a44d402de3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Undefined-2 These authors contributed equally to this work. These authors also contributed equally to this work. |
ORCID | 0000-0002-8612-6469 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192581/ |
PMID | 32352379 |
PQID | 2407982978 |
PQPubID | 2045579 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_445809e39596411894c241e66afcfafe pubmedcentral_primary_oai_pubmedcentral_nih_gov_7192581 proquest_miscellaneous_2396854783 proquest_journals_2407982978 crossref_primary_10_7554_eLife_51912 pubmed_primary_32352379 |
PublicationCentury | 2000 |
PublicationDate | 2020-04-30 |
PublicationDateYYYYMMDD | 2020-04-30 |
PublicationDate_xml | – month: 04 year: 2020 text: 2020-04-30 day: 30 |
PublicationDecade | 2020 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: Cambridge |
PublicationTitle | eLife |
PublicationTitleAlternate | Elife |
PublicationYear | 2020 |
Publisher | eLife Sciences Publications Ltd eLife Sciences Publications, Ltd |
Publisher_xml | – sequence: 0 name: eLife Sciences Publications Ltd – name: eLife Sciences Publications, Ltd – name: eLife Sciences Publications Ltd |
References | 32484778 - Elife. 2020 Jun 02;9 Kopanska (bib29) 2016; 11 Huijbers (bib23) 2010; 5 Dembo (bib10) 1999; 76 Han (bib21) 2018; 115 Trepat (bib45) 2009; 5 Legant (bib31) 2010; 7 Xu (bib48) 2015; 92 Carey (bib4) 2013; 30 Kopanska (bib28) 2015; 125 Pointer (bib38) 2017; 126 Kaufman (bib25) 2005; 89 Holle (bib22) 2018; 18 Böhringer (bib1) 2020 Koch (bib27) 2012; 7 Chen (bib6) 2019; 84 Cóndor (bib7) 2017; 75 Shipitsin (bib39) 2007; 11 Tambe (bib43) 2011; 10 Hall (bib19) 2016; 113 Steinwachs (bib40) 2015 Friedrich (bib14) 2009; 4 Burkel (bib2) 2017 Levental (bib32) 2007; 3 Morley (bib33) 2019; 10 Chaudhuri (bib5) 2018; 118 Friedl (bib13) 2003; 3 Valencia (bib46) 2015; 6 Dolega (bib11) 2017; 8 Storm (bib42) 2005; 435 Nunes (bib35) 2019; 116 Gerum (bib15) 2019 Hunter (bib24) 2007; 9 Taylor (bib44) 2010; 59 Steinwachs (bib41) 2016; 13 Geuzaine (bib17) 2009; 79 Weigand (bib47) 2016; 18 Cuddapah (bib9) 2014; 15 Münster (bib34) 2013; 110 Cox (bib8) 2011; 4 Han (bib20) 2016; 113 Grimmer (bib18) 2018; 140 Lee (bib30) 2017; 7 Friedl (bib12) 2009; 10 Butler (bib3) 2002; 282 Gerum (bib16) 2020 Kim (bib26) 2017; 8 Otsu (bib36) 1979; 9 Payne (bib37) 2013; 11 |
References_xml | – volume: 8 year: 2017 ident: bib26 article-title: Stress-induced plasticity of dynamic collagen networks publication-title: Nature Communications doi: 10.1038/s41467-017-01011-7 contributor: fullname: Kim – volume: 7 year: 2017 ident: bib30 article-title: Local alignment vectors reveal Cancer cell-induced ECM fiber remodeling dynamics publication-title: Scientific Reports doi: 10.1038/srep39498 contributor: fullname: Lee – volume: 4 start-page: 309 year: 2009 ident: bib14 article-title: Spheroid-based drug screen: considerations and practical approach publication-title: Nature Protocols doi: 10.1038/nprot.2008.226 contributor: fullname: Friedrich – volume: 3 start-page: 299 year: 2007 ident: bib32 article-title: Soft biological materials and their impact on cell function publication-title: Soft Matter doi: 10.1039/B610522J contributor: fullname: Levental – volume: 110 start-page: 12197 year: 2013 ident: bib34 article-title: Strain history dependence of the nonlinear stress response of fibrin and collagen networks publication-title: PNAS doi: 10.1073/pnas.1222787110 contributor: fullname: Münster – volume: 13 start-page: 171 year: 2016 ident: bib41 article-title: Three-dimensional force microscopy of cells in biopolymer networks publication-title: Nature Methods doi: 10.1038/nmeth.3685 contributor: fullname: Steinwachs – volume: 8 start-page: 1 year: 2017 ident: bib11 article-title: Cell-like pressure sensors reveal increase of mechanical stress towards the core of multicellular spheroids under compression publication-title: Nature Communications doi: 10.1038/ncomms14056 contributor: fullname: Dolega – volume: 435 start-page: 191 year: 2005 ident: bib42 article-title: Nonlinear elasticity in biological gels publication-title: Nature doi: 10.1038/nature03521 contributor: fullname: Storm – volume: 115 start-page: 4075 year: 2018 ident: bib21 article-title: Cell contraction induces long-ranged stress stiffening in the extracellular matrix publication-title: PNAS doi: 10.1073/pnas.1722619115 contributor: fullname: Han – volume: 126 start-page: 1812 year: 2017 ident: bib38 article-title: Association of collagen architecture with glioblastoma patient survival publication-title: Journal of Neurosurgery doi: 10.3171/2016.6.JNS152797 contributor: fullname: Pointer – volume: 18 start-page: 1 year: 2016 ident: bib47 article-title: Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells publication-title: Breast Cancer Research doi: 10.1186/s13058-016-0688-2 contributor: fullname: Weigand – volume: 89 start-page: 635 year: 2005 ident: bib25 article-title: Glioma expansion in collagen I matrices: analyzing collagen concentration-dependent growth and motility patterns publication-title: Biophysical Journal doi: 10.1529/biophysj.105.061994 contributor: fullname: Kaufman – year: 2017 ident: bib2 article-title: Quantitative image analysis for investigating Cell-Matrix interactions contributor: fullname: Burkel – volume: 5 year: 2010 ident: bib23 article-title: A role for fibrillar collagen deposition and the collagen internalization receptor endo180 in glioma invasion publication-title: PLOS ONE doi: 10.1371/journal.pone.0009808 contributor: fullname: Huijbers – volume: 282 start-page: C595 year: 2002 ident: bib3 article-title: Traction fields, moments, and strain energy that cells exert on their surroundings publication-title: American Journal of Physiology-Cell Physiology doi: 10.1152/ajpcell.00270.2001 contributor: fullname: Butler – volume: 118 start-page: 6499 year: 2018 ident: bib5 article-title: Mechanobiology of tumor growth publication-title: Chemical Reviews doi: 10.1021/acs.chemrev.8b00042 contributor: fullname: Chaudhuri – volume: 75 year: 2017 ident: bib7 article-title: Traction force microscopy in 3-Dimensional extracellular matrix networks publication-title: Current Protocols in Cell Biology doi: 10.1002/cpcb.24 contributor: fullname: Cóndor – volume: 84 start-page: 280 year: 2019 ident: bib6 article-title: Early stage mechanical remodeling of collagen surrounding head and neck squamous cell carcinoma spheroids correlates strongly with their invasion capability publication-title: Acta Biomaterialia doi: 10.1016/j.actbio.2018.11.046 contributor: fullname: Chen – year: 2015 ident: bib40 article-title: Cellular Forces during Migration through Collagen Networks contributor: fullname: Steinwachs – volume: 4 start-page: 165 year: 2011 ident: bib8 article-title: Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and Cancer publication-title: Disease Models & Mechanisms doi: 10.1242/dmm.004077 contributor: fullname: Cox – volume: 15 start-page: 455 year: 2014 ident: bib9 article-title: A neurocentric perspective on glioma invasion publication-title: Nature Reviews Neuroscience doi: 10.1038/nrn3765 contributor: fullname: Cuddapah – volume: 10 start-page: 469 year: 2011 ident: bib43 article-title: Collective cell guidance by cooperative intercellular forces publication-title: Nature Materials doi: 10.1038/nmat3025 contributor: fullname: Tambe – volume: 5 start-page: 426 year: 2009 ident: bib45 article-title: Physical forces during collective cell migration publication-title: Nature Physics doi: 10.1038/nphys1269 contributor: fullname: Trepat – volume: 113 start-page: 11208 year: 2016 ident: bib20 article-title: Oriented collagen fibers direct tumor cell intravasation publication-title: PNAS doi: 10.1073/pnas.1610347113 contributor: fullname: Han – volume: 7 year: 2012 ident: bib27 article-title: 3d traction forces in Cancer cell invasion publication-title: PLOS ONE doi: 10.1371/journal.pone.0033476 contributor: fullname: Koch – volume-title: GitHub year: 2020 ident: bib16 article-title: saenopy contributor: fullname: Gerum – volume: 7 start-page: 969 year: 2010 ident: bib31 article-title: Measurement of mechanical tractions exerted by cells in three-dimensional matrices publication-title: Nature Methods doi: 10.1038/nmeth.1531 contributor: fullname: Legant – volume: 116 start-page: 206 year: 2019 ident: bib35 article-title: 3d tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs publication-title: Biotechnology and Bioengineering doi: 10.1002/bit.26845 contributor: fullname: Nunes – volume-title: GitHub year: 2020 ident: bib1 article-title: A Python package to conduct 3D Traction Force Microscopy on multicellular aggregates (spheroids) contributor: fullname: Böhringer – volume: 76 start-page: 2307 year: 1999 ident: bib10 article-title: Stresses at the cell-to-substrate interface during locomotion of fibroblasts publication-title: Biophysical Journal doi: 10.1016/S0006-3495(99)77386-8 contributor: fullname: Dembo – volume: 125 start-page: 353 year: 2015 ident: bib28 article-title: Quantification of collagen contraction in three-dimensional cell culture publication-title: Methods in Cell Biology doi: 10.1016/bs.mcb.2014.10.017 contributor: fullname: Kopanska – volume: 6 start-page: 43438 year: 2015 ident: bib46 article-title: Collective cancer cell invasion induced by coordinated contractile stresses publication-title: Oncotarget doi: 10.18632/oncotarget.5874 contributor: fullname: Valencia – volume: 11 year: 2016 ident: bib29 article-title: Tensile forces originating from Cancer spheroids facilitate tumor invasion publication-title: PLOS ONE doi: 10.1371/journal.pone.0156442 contributor: fullname: Kopanska – volume: 113 start-page: 14043 year: 2016 ident: bib19 article-title: Fibrous nonlinear elasticity enables positive mechanical feedback between cells and ECMs publication-title: PNAS doi: 10.1073/pnas.1613058113 contributor: fullname: Hall – volume: 11 start-page: 259 year: 2007 ident: bib39 article-title: Molecular Definition of Breast Tumor Heterogeneity publication-title: Cancer Cell doi: 10.1016/j.ccr.2007.01.013 contributor: fullname: Shipitsin – volume: 30 start-page: 615 year: 2013 ident: bib4 article-title: Leading malignant cells initiate collective epithelial cell invasion in a three-dimensional heterotypic tumor spheroid model publication-title: Clinical & Experimental Metastasis doi: 10.1007/s10585-013-9565-x contributor: fullname: Carey – volume: 59 start-page: 3262 year: 2010 ident: bib44 article-title: Long-Duration Time-Resolved PIV to study unsteady aerodynamics publication-title: IEEE Transactions on Instrumentation and Measurement doi: 10.1109/TIM.2010.2047149 contributor: fullname: Taylor – volume: 3 start-page: 362 year: 2003 ident: bib13 article-title: Tumour-cell invasion and migration: diversity and escape mechanisms publication-title: Nature Reviews Cancer doi: 10.1038/nrc1075 contributor: fullname: Friedl – volume: 18 start-page: 1 year: 2018 ident: bib22 article-title: Cell-Extracellular matrix mechanobiology: forceful tools and emerging needs for basic and translational research publication-title: Nano Letters doi: 10.1021/acs.nanolett.7b04982 contributor: fullname: Holle – volume-title: arXiv year: 2019 ident: bib15 article-title: Pylustrator: code generation for reproducible figures for publication contributor: fullname: Gerum – volume: 92 year: 2015 ident: bib48 article-title: Nonlinearities of biopolymer gels increase the range of force transmission publication-title: Physical Review E doi: 10.1103/PhysRevE.92.032728 contributor: fullname: Xu – volume: 10 start-page: 1 year: 2019 ident: bib33 article-title: Quantitative characterization of 3D bioprinted structural elements under cell generated forces publication-title: Nature Communications doi: 10.1038/s41467-019-10919-1 contributor: fullname: Morley – volume: 10 start-page: 445 year: 2009 ident: bib12 article-title: Collective cell migration in morphogenesis, regeneration and cancer publication-title: Nature Reviews Molecular Cell Biology doi: 10.1038/nrm2720 contributor: fullname: Friedl – volume: 140 year: 2018 ident: bib18 article-title: Displacement propagation and nonaffinity in collagen networks due to local contraction publication-title: Journal of Biomechanical Engineering doi: 10.1115/1.4038744 contributor: fullname: Grimmer – volume: 9 start-page: 90 year: 2007 ident: bib24 article-title: Matplotlib: a 2D graphics environment publication-title: Computing in Science & Engineering doi: 10.1109/MCSE.2007.55 contributor: fullname: Hunter – volume: 9 start-page: 62 year: 1979 ident: bib36 article-title: A threshold selection method from Gray-Level histograms publication-title: IEEE Transactions on Systems, Man, and Cybernetics doi: 10.1109/TSMC.1979.4310076 contributor: fullname: Otsu – volume: 79 start-page: 1309 year: 2009 ident: bib17 article-title: Gmsh: a 3-D finite element mesh generator with built-in pre- and post-processing facilities publication-title: International Journal for Numerical Methods in Engineering doi: 10.1002/nme.2579 contributor: fullname: Geuzaine – volume: 11 start-page: 1129 year: 2013 ident: bib37 article-title: The pathobiology of collagens in glioma publication-title: Molecular Cancer Research doi: 10.1158/1541-7786.MCR-13-0236 contributor: fullname: Payne |
SSID | ssj0000748819 |
Score | 2.4969027 |
Snippet | We describe a method for quantifying the contractile forces that tumor spheroids collectively exert on highly nonlinear three-dimensional collagen networks.... |
SourceID | doaj pubmedcentral proquest crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
SubjectTerms | biopolymer gels Biopolymers Breast cancer Breast Neoplasms - metabolism Breast Neoplasms - pathology Cancer Biology Cell Culture Techniques Cell Line, Tumor Cell Shape Cell size Collagen Collagen - chemistry Collagen - metabolism collective force generation Computer Simulation Contractility Deformation Experiments Extracellular matrix Female Gels Glioblastoma - metabolism Glioblastoma - pathology Humans Mechanotransduction, Cellular Microscopy Microscopy, Video Models, Biological multicellular aggregates Physics of Living Systems Polymerization Protein Conformation Spheroids Spheroids, Cellular Stress, Mechanical Time-Lapse Imaging Tools and Resources traction force microscopy Tumor Cells, Cultured tumor spheroids tumoroids |
SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS_QwFA3iyo344at-KhHcVptX0yxVFBF1peCu5HGLXdjKTGfhv_cmHYcZEdy4bJNFcm5ucg5JTgg5NYZD4yqRO1nIXHpf5baM3njCMxGE8c7Hi8IPj-Xts7x7US9LT33FM2GjPfAI3LmUqioMCKNMKZENG-lx0YGytI1vbANp9i34kphKc7DGgcnMeCFP45J5DvdtA2fIVxhfWYKSU_9P9PL7KcmlZedmi2zO-SK9GNv5j6xBt00ektxPMxVF0ompTvuGDrO3fkKn0Segb8OUth0dMFKQh-jgP7pvUNfGVxE-3mBCu_EE-HSHPN9cP13d5vN3EXKvmB7yyjnfaKmUlYW1nlsEQyoPrsJPjYoNWQcLpUUyA4iRAKVCEZiVMqBaDCB2yXrXd7BPqGZOgTFeWBRWUGrLZWgw5Tm3KB2YycjpF1T1-2h_UaNsiIjWCdE6IZqRywjjokr0rE4_MJL1PJL1b5HMyOFXEOp5Ik3rKDhNvP5bZeRkUYwpEPc1bAf9DOsIU1bRl0xkZG-M2aIlgiPDFBo7oleiudLU1ZKufU022xrJr6rYwV_07T_Z4FGop22oQ7I-TGZwhGxmcMdp4H4CgNX1EQ priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELZKe-GCiigQWpCReg1dv2L7VBVEVSHKqZX2FvkxgRyatJvsof-esZPdsghxTOLDZMbj-T4_PhNyai2HxhtRermQpQzBlK5K2ngiMBGFDT6kg8LXP6qrW_ltqZbzhNswb6vcjIl5oI59SHPkZ4l52HQO1JzfP5Tp1qi0ujpfofGMHDCOpRz7s17q7RwLlkeDFW86lqexcJ7B97aBT4haGN8pRFmv_18g8--9kn8Un8tD8mJGjfRiCvNLsgfdK3KdSX8eryhCT0x42jd0XN_1KzoktYC-jQNtOzpivKCMScd_0uCgvk13IzzewYp20z7w4YjcXn69-XJVzrcjlEExPZbG-9BoqZSTC-cCd1XlpArgDT5q5G2IPVisHEIaCI0ToFRcROakjMgZI4jXZL_rO3hLqGZegbVBOKRXUGnHZWww8Tl3SCCYLcjpxlX1_SSCUSN5SB6ts0fr7NGCfE5u3DZJytX5Rb_6Wc-JUEupzMKCsMpWEtmNlQFBBKDxDVrZQEFONkGo53Qa6qfgF-Tj9jMmQlrdcB30a2wjbGWSOpkoyJspZltLBEecKTT-iN6J5o6pu1-69lcW29YIgZVh7_5v1jF5zhMRz8tMJ2R_XK3hPaKV0X_IXfI3EmztLw priority: 102 providerName: ProQuest – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3PT9swFLYQXHZBQ7AtW5mMxDVdE9txfEIwUVXTutMqcYv84wUi0QTSVFr_e56dtKKIw45JHMV5z8_v-2T7e4RcKpVCaXIWGz7hMbc2j3XmtfGYTZhjyhrrDwrP_2SzBf91J-4OyLYY52DA1bvUzteTWrSP43_PmysMeMSvY4nZ8Af8rkoYIxTx1YaPUkyJfm_XfMD5YUqWOE4T1Z_Pe_vOXkYKwv3voc23myZfZaHpR3I8wEd63fv7hBxAfUrmgf2HiYsiBsXIp01Ju_WyaenKywY0lVvRqqYdOg5i5wX9ezEOaipfJGGzhJbW_Ybw1RlZTG___pzFQ5mE2IpEdnFujC0lF0LzidY21VmmubBgcryUSOAQhCQu04htwJaagRBu4hLNuUPy6IB9Iod1U8MXQmViBChlmUaeBZnUKXclzgBpqpFJJCoil1tTFU-9GkaBLMJbtAgWLYJFI3Ljzbhr4iWsw42mvS-GiCg4F_lEAVNCZRxpjuIW0QRg50vsZQkRGW2dUGyHReH5p_KngfOIXOweY0T4ZQ5dQ7PGNkxluZcpYxH53Pts1xOWIuBkEn9E7nlzr6v7T-rqIahuS8TCIk--_sd3v5EPqaflYdFpRA67dg3niF068z2Myxf61fGr priority: 102 providerName: Scholars Portal |
Title | Collective forces of tumor spheroids in three-dimensional biopolymer networks |
URI | https://www.ncbi.nlm.nih.gov/pubmed/32352379 https://www.proquest.com/docview/2407982978 https://search.proquest.com/docview/2396854783 https://pubmed.ncbi.nlm.nih.gov/PMC7192581 https://doaj.org/article/445809e39596411894c241e66afcfafe |
Volume | 9 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pa9swFH603WWXsdH9cNcFFXp1ElmSJR3X0lJKU8pYITcjyfJmWOySOIf993uS7ZCMnXYx2JLh8d6T9X3W0yeAS60zX1nFUsvnPOXOqdTkQRuPOcpKpp11YaPw4jG_e-b3S7E8AjHuhYlF-87W0-bXatrUP2Nt5cvKzcY6sdnT4loiLBGKzo7hGKffPYoeP78Sc5Lqfi-exNly5h_qyk8RqtBwdg3LEHOwULq1NxFFvf5_gcy_ayX3Jp_bt_BmQI3ka2_dOzjyzSksIumP3yuC0BMHPGkr0m1X7ZpsglpAW5cbUjekw3j5tAw6_r0GB7F1OBvh98qvSdPXgW_ew_Ptzffru3Q4HSF1gsouVda6SnIhDJ8b4zKT54YL563CW4m8DbEHLXODkMa7yjAvRDkvqeG8RM5YevYBTpq28Z-ASGqF19oxg_TK59JkvKxw4GeZQQJBdQKXo6uKl14Eo0DyEJxbROcW0bkJXAU37roE5er4oF3_KIb4FZwLNdeeaaFzjuxGc4cgwqPxFVpZ-QTOxyAUw3DaFIF26rAJWCVwsWvGgRBWN0zj2y32YTpXQZ2MJfCxj9nOkjHmCciDaB6YetiCuRfFtodcO_vvNz_D6yxw9LgCdQ4n3XrrvyCQ6ewE03cpJ_Dq6ubx6dsk_g7A64KrSUzpPykC-mU |
link.rule.ids | 230,314,727,780,784,864,885,2102,12056,21388,24318,27924,27925,31719,31720,33744,33745,43310,43805,53791,53793,73745,74302 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwELVge4ALouIrtBQj9Rq6ie3YPiGKWi2wu0KolXqzHHtScmhSNtlD_z1jJ7t0UcUxsQ-TmYznPX88E3KsdQ5VqVha8ilPuXMqtUXQxmMuY55pV7pwUHixLGaX_NuVuBon3LpxW-VmTIwDtW9dmCM_CcxDh3Og6tPt7zTcGhVWV8crNB6TvaCcLiZk7_Rs-ePndpYFC6TCmjcczJNYOk9gXlfwEXFLlu-UoqjY_xDM_He35L3yc_6cPBtxI_08BHqfPILmBVlE2h9HLIrgE1OethXt1zftinZBL6CtfUfrhvYYMUh9UPIfVDhoWYfbEe5uYEWbYSd495Jcnp9dfJml4_0IqROZ7FNVlq6SXAjLp9a63BaF5cJBqfBRInND9JH5wiKoAVdZBkL4qc8s5x5Zowf2ikyatoE3hMqsFKC1YxYJFhTS5txXmPp5bpFCZDohxxtXmdtBBsMgfQgeNdGjJno0IafBjdsuQbs6vmhX12ZMBcO5UFMNTAtdcOQ3mjuEEYDGV2hlBQk53ATBjAnVmb_hT8iHbTOmQljfsA20a-zDdKGCPhlLyOshZltLWI5Ik0n8ELkTzR1Td1ua-leU25YIgoXK3v7frPfkyexiMTfzr8vvB-RpHmh5XHQ6JJN-tYZ3iF368mj8Qf8AZr7xhQ |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELZgKyEuFRWvlFKM1GvYxI8kPiEKXRVoVxWiUm-RH2PIoUnZZA_8e8aJd2ER4pjEh8mMZ-Ybe_yZkBOlGHhT8dSITKTC2irVReDG4zbnjitrbDgofLkszq_Fpxt5E_uf-thWuYmJY6B2nQ1r5PNQeahwDrSa-9gWcfVh8fbuRxpukAo7rfE6jftkD7NixmZk7_RsefVlu-KCybLC_Dcd0isxjc7hovHwBjFMznbS0sje_y_I-Xfn5B-paPGI7EcMSd9NRj8g96B9TC7HJYAxelEEouj-tPN0WN92K9oH7oCucT1tWjqg9SB1gdV_YuSgpgk3Jfy8hRVtp67w_gm5Xpx9fX-exrsSUivzckgrY6wvhZRaZFpbpotCC2nBVPhYYhWHSCR3hUaAA9ZrDlK6zOVaCIcVpAP-lMzaroXnhJa5kaCU5RqLLShKzYTzGAYY01hO5CohJxtV1XcTJUaNpUTQaD1qtB41mpDToMbtkMBjPb7oVt_q6Ba1ELLKFHAlVSGw1lHCIqQAFN6jlB4ScrQxQh2dq69_T4WEvN5-RrcIex26hW6NY7gqqsBVxhPybLLZVhLOEHXyEn-k3LHmjqi7X9rm-0i9XSIgllV--H-xXpEHODfri4_Lzy_IQxYq9HH_6YjMhtUaXiKMGcxxnJ-_AGKV9bI |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Collective+forces+of+tumor+spheroids+in+three-dimensional+biopolymer+networks&rft.jtitle=eLife&rft.au=Mark%2C+Christoph&rft.au=Grundy%2C+Thomas+J&rft.au=Strissel%2C+Pamela+L&rft.au=B%C3%B6hringer%2C+David&rft.date=2020-04-30&rft.eissn=2050-084X&rft.volume=9&rft_id=info:doi/10.7554%2FeLife.51912&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2050-084X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2050-084X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2050-084X&client=summon |