A rapid novel visualized loop-mediated isothermal amplification method for Salmonella detection targeting at fimW gene

• Published in: Poultry science Vol. 99; no. 7; pp. 3637 - 3642
• Main Authors: Wen, Junping, Gou, Hongchao, Zhan, Zeqiang, Gao, Yuan, Chen, Zhengquan, Bai, Jie, Wang, Shaojun, Chen, Kaifeng, Lin, Qijie, Liao, Ming, Zhang, Jianmin
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.07.2020; Elsevier
• Subjects: Animals; Bacterial Proteins - analysis; Chickens; DNA-Binding Proteins - analysis; Ducks; LAMP; Microbiology and Food Safety; Molecular Diagnostic Techniques - instrumentation; Molecular Diagnostic Techniques - methods; novel visualized detection; Nucleic Acid Amplification Techniques - instrumentation; Nucleic Acid Amplification Techniques - methods; Poultry Diseases - diagnosis; Salmonella enterica - genetics; Salmonella enterica - isolation & purification; Salmonella Infections, Animal - diagnosis; Salmonella, fimW gene; Sensitivity and Specificity; novel visualized detection; Salmonella, fimW gene; LAMP
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2020.03.045

Salmonella infection causes huge losses in the poultry industry worldwide. With the aim to prevent infectious diseases caused by Salmonella and to achieve rapid visualized Salmonella detection in poultry production, we used cresol red as an indicator to develop a novel visualized loop-mediated isothermal amplification method that targets the Salmonella fimW gene firstly in related field. The detection limit was 7.3 × 101 CFU/mL, and the method was highly specific and showed a high clinical detection rate. The entire reaction can be completed in about 40 min and only requires a water bath at 62°C, which makes the method extremely suitable for application to poultry production.