An algorithm for the typing of enteroviruses and correlation to serotyping by viral neutralization

• Published in: Journal of clinical virology Vol. 45; no. 4; pp. 334 - 340
• Main Authors: Kiang, David, Newbower, Elly Chou, Yeh, Elaine, Wold, Lauren, Chen, Lily, Schnurr, David P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.2009; Elsevier
• Subjects: Algorithms; Allergy and Immunology; Antigens, Viral - analysis; Biological and medical sciences; Cluster Analysis; Enterovirus; Enterovirus - classification; Enterovirus - genetics; Enterovirus - immunology; Fluorescent Antibody Technique, Direct - methods; Fundamental and applied biological sciences. Psychology; Genotype; Genotyping; Hepatitis E virus; Human viral diseases; Humans; Immunofluorescence; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Molecular Sequence Data; Neutralization Tests; Phylogenetics; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology; Serotyping; Viral diseases; Virology; Phylogenetics; Enterovirus; Genotyping; Immunofluorescence; Typing; Microbiology; Picornaviridae; Genotype; Phylogeny; Algorithm; Virology; Virus; Serotype; Neutralization

Human enteroviruses (HEVs) are common pathogens which cause a broad spectrum of illnesses ranging from asymptomatic infection to acute myocarditis and aseptic meningitis. The neutralization assay for serotype determination is labor-intensive and time-consuming. There is a need for a methodology that...