A stress-free strategy to correct point mutations in patient iPS cells

• Published in: Stem cell research Vol. 53; p. 102332
• Main Authors: Cai, Jingli, Kropf, Elizabeth, Hou, Ya-Ming, Iacovitti, Lorraine
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.05.2021; Elsevier
• Subjects: Clone Cells; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas Systems - genetics; CRISPR-Cas9; Gene targeting; Homologous Recombination; Humans; Induced Pluripotent Stem Cells; Isogenic iPS cells; Mutation; Point Mutation; Isogenic iPS cells; Gene targeting; CRISPR-Cas9; Point mutation

•A CRISPR-Cas9 gene knock-in method for correction of point mutations or frameshifting.•sgRNA sequences selected from the nearest intron of the mutation providing more flexibility.•Donor vectors containing correct genomic DNA information on the homologous arms and antibiotic selection cassette.•No o...