Functional and molecular effects of TNF-α on human iPSC-derived cardiomyocytes

• Published in: Stem cell research Vol. 52; p. 102218
• Main Authors: Saraf, Anita, Rampoldi, Antonio, Chao, Myra, Li, Dong, Armand, Lawrence, Hwang, Hyun, Liu, Rui, Jha, Rajnesh, Fu, Haian, Maxwell, Joshua T., Xu, Chunhui
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.04.2021; Elsevier
• Subjects: Ca2+ transients; Ca2+propagation; Calcium; Cardiomyocytes; Cell Differentiation; Cytokines; Human iPSC; Humans; Induced Pluripotent Stem Cells; Myocytes, Cardiac; TNFα; Tumor Necrosis Factor-alpha; Cardiomyocytes; TNFα; Cytokines; Ca2+ transients; Ca2+propagation; Human iPSC; Ca(2+)propagation; Ca(2+) transients

Treatment of human iPSC-cardiomyocytes with proinflammatory cytokine TNF-α resulted in increased ROS, caspase activity and cell death, as well as abnormal calcium handling and abnormal contractility. Numerous molecular pathways were affected that correlated to cardiomyocyte function, including up-re...