Epigenetic regulation of RELN and GAD1 in the frontal cortex (FC) of autism spectrum disorder (ASD) subjects

• Published in: International journal of developmental neuroscience Vol. 62; no. 1; pp. 63 - 72
• Main Authors: Zhubi, Adrian, Chen, Ying, Guidotti, Alessandro, Grayson, Dennis R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.11.2017; Elsevier BV
• Subjects: 5-Methylcytosine - analogs & derivatives; 5-Methylcytosine - pharmacology; Adolescent; Adult; Analysis of Variance; Autism; Autism Spectrum Disorder - genetics; Autism Spectrum Disorder - pathology; Brain; Cell Adhesion Molecules, Neuronal - genetics; Cell Adhesion Molecules, Neuronal - metabolism; Chromatin; Chromatin Immunoprecipitation; Cohort Studies; Cortex (frontal); Deoxyribonucleic acid; DNA; DNA (Cytosine-5-)-Methyltransferase 1 - metabolism; DNMT1 protein; Epigenesis, Genetic - physiology; Epigenetics; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Female; Frontal Lobe - metabolism; GABA neuron; Gene expression; Gene regulation; Genes; Glutamate decarboxylase; Glutamate Decarboxylase - genetics; Glutamate Decarboxylase - metabolism; Humans; Hydroxymethylcytosine; Immunoprecipitation; Male; MeCP2 protein; Methyl-CpG binding protein; Methyl-CpG-Binding Protein 2 - metabolism; Methylcytosine; mRNA; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurons; Promoter Regions, Genetic; Promoters; Protein Binding - genetics; Proteins; Reelin Protein; Repressors; Ribonucleic acid; RNA; RNA, Messenger - metabolism; Serine Endopeptidases - genetics; Serine Endopeptidases - metabolism; Transcription; Translocation; Young Adult; γ-Aminobutyric acid; GABRB3; DNMT1; OXTR; TET; Chromatin; Transcription; 5caC; ChIP; ACTB; 5hmC; MECP2; 5fC; ASD; RELN; Repressors; RETT; BDNF-Tot; CB; MeDIP; Hydroxymethylcytosine; CON; BDNF; GAD1; IP; qRT-PCR; GAD2; 5mC; Gene expression; hMeDIP; BDNF-IV; BDNF-IX; REST; Methylcytosine; GABA; GABA neuron; FC; GAPDH; BA

•The RELN and GAD1 mRNA levels were reduced in ASD PFC.•TET 1, 2, and 3 mRNAs were increased in ASD PFC.•There was increased DNMT1 and MECP2 promoter binding.•5mC and 5hmC levels in the promoters do not account for the increased binding. These data and a study on the cerebella from the same ASD coho...