Optimization of Zebrafish Larvae Sectioning for Mass Spectrometry Imaging
The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, a...
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| Published in | Pharmaceuticals (Basel, Switzerland) Vol. 15; no. 10; p. 1230 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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Basel
MDPI AG
01.10.2022
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| Abstract | The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. |
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| AbstractList | The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae.The utility of zebrafish is becoming more frequent due to lower costs and high similarities to humans. Zebrafish larvae are attractive subjects for drug screening and drug metabolism research. However, obtaining good quality zebrafish larvae sections for batch samples at designated planes, angles, and locations for comparison purposes is a challenging task. We report here the optimization of fresh frozen zebrafish larvae sectioning for mass spectrometry imaging. We utilized the gelatin solutions that were created at two different temperatures (50 and 85 °C) as embedding media. Gelatin-50 (gelatin created under 50 °C, solid gel under room temperature) was used to make a larvae-shaped mold and gelatin-85 (gelatin created under 85 °C, liquid under room temperature) was used to embed the larvae. H&E staining of sections shows well-preserved morphology and minimal histological interference. More importantly, the position of the larvae was well controlled resulting in more consistent sectioning of the larvae. |
| Audience | Academic |
| Author | Yang, Junhai Sawicki, James Talaty, Nari Buck, Wayne Cassar, Steven Wagner, David Rendino, Lauren |
| AuthorAffiliation | AbbVie Inc., 1 Waukegan Rd., North Chicago, IL 60064, USA |
| AuthorAffiliation_xml | – name: AbbVie Inc., 1 Waukegan Rd., North Chicago, IL 60064, USA |
| Author_xml | – sequence: 1 givenname: Junhai surname: Yang fullname: Yang, Junhai – sequence: 2 givenname: Lauren orcidid: 0000-0002-5531-9313 surname: Rendino fullname: Rendino, Lauren – sequence: 3 givenname: Steven surname: Cassar fullname: Cassar, Steven – sequence: 4 givenname: Wayne surname: Buck fullname: Buck, Wayne – sequence: 5 givenname: James surname: Sawicki fullname: Sawicki, James – sequence: 6 givenname: Nari surname: Talaty fullname: Talaty, Nari – sequence: 7 givenname: David surname: Wagner fullname: Wagner, David |
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| Cites_doi | 10.1021/ac200998a 10.1177/0957154X07070335 10.1016/j.reprotox.2011.06.121 10.1007/s00216-021-03476-4 10.1093/nar/gkm324 10.1007/s00216-020-03128-z 10.1038/nprot.2010.165 10.1021/acs.analchem.0c00826 10.1002/jms.505 10.1016/j.toxlet.2014.06.201 10.1002/rcm.4401 10.20517/2572-8180.2017.15 10.1021/ac970888i 10.1007/s13361-016-1422-0 10.1021/ac202383m 10.7171/jbt.13-2403-002 10.1016/j.clinms.2019.01.004 10.3390/molecules25173974 10.1038/s41598-017-17204-5 10.1016/j.foodhyd.2022.107535 10.1101/376657 10.1007/s13361-015-1132-z 10.1194/jlr.R800095-JLR200 10.1021/acs.chemrestox.9b00335 10.1016/j.foodhyd.2019.01.059 10.1016/j.scitotenv.2021.152621 10.1021/acs.analchem.7b04733 |
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| SubjectTerms | Analysis Drug metabolism embedding medium Embryos Health aspects MALDI imaging mass spectrometry Mass spectrometry Medical care, Cost of Scientific imaging sectioning Viscosity Zebrafish zebrafish larvae |
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