Optimization of Sources of Circulating Cell-Free DNA Variability for Downstream Molecular Analysis

• Published in: The Journal of molecular diagnostics : JMD Vol. 23; no. 11; pp. 1545 - 1552
• Main Authors: Till, Jacob E., Black, Taylor A., Gentile, Caren, Abdalla, Aseel, Wang, Zhuoyang, Sangha, Hareena K., Roth, Jacquelyn J., Sussman, Robyn, Yee, Stephanie S., O'Hara, Mark H., Thompson, Jeffrey C., Aggarwal, Charu, Hwang, Wei-Ting, Elenitoba-Johnson, Kojo S.J., Carpenter, Erica L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2021; American Society for Investigative Pathology
• Subjects: Alleles; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Blood Specimen Collection - methods; Carcinoma, Pancreatic Ductal - blood; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - pathology; Case-Control Studies; Circulating Tumor DNA - blood; Circulating Tumor DNA - genetics; Circulating Tumor DNA - isolation & purification; Cohort Studies; Humans; Molecular Diagnostic Techniques - methods; Mutation; Neoplasm Metastasis; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Real-Time Polymerase Chain Reaction - methods; Regular

Circulating cell-free DNA (ccfDNA) is used increasingly as a cancer biomarker for prognostication, as a correlate for tumor volume, or as input for downstream molecular analysis. Determining optimal blood processing and ccfDNA quantification are crucial for ccfDNA to serve as an accurate biomarker a...