Massively Parallel Sequencing of Esophageal Brushings Enables an Aneuploidy-Based Classification of Patients With Barrett’s Esophagus

• Published in: Gastroenterology (New York, N.Y. 1943) Vol. 160; no. 6; pp. 2043 - 2054.e2
• Main Authors: Douville, Christopher, Moinova, Helen R., Thota, Prashanthi N., Shaheen, Nicholas J., Iyer, Prasad G., Canto, Marcia Irene, Wang, Jean S., Dumot, John A., Faulx, Ashley, Kinzler, Kenneth W., Papadopoulos, Nickolas, Vogelstein, Bert, Markowitz, Sanford D., Bettegowda, Chetan, Willis, Joseph E., Chak, Amitabh
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2021
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - pathology; Aneuploidy; Barrett Esophagus - classification; Barrett Esophagus - genetics; Barrett Esophagus - pathology; Barrett’s Esophagus; Chromosome 8q; Cross-Sectional Studies; Cytological Techniques; Disease Progression; Esophageal Neoplasms - genetics; Esophageal Neoplasms - pathology; Esophagus - pathology; High-Throughput Nucleotide Sequencing; Humans; RealSeqS; WGS; Barrett’s Esophagus; BE; HGD; BAD; WALDO; EAC; RealSeqS; LGD; Aneuploidy; SVM; Chromosome 8q; CRD; GAS; NDBE

Aneuploidy has been proposed as a tool to assess progression in patients with Barrett’s esophagus (BE), but has heretofore required multiple biopsies. We assessed whether a single esophageal brushing that widely sampled the esophagus could be combined with massively parallel sequencing to characteri...