Specificity-determining regions of a lepidopteran-specific insecticidal protein produced by Bacillus thuringiensis

• Published in: The Journal of biological chemistry Vol. 265; no. 34; pp. 20923 - 20930
• Main Authors: Schnepf, H E, Tomczak, K, Ortega, J P, Whiteley, H R
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.12.1990; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; AMINO ACID SEQUENCES; Animals; BACILLUS THURINGIENSIS; Bacillus thuringiensis - genetics; Bacillus thuringiensis Toxins; BACTERIA; Bacterial Proteins - genetics; Bacterial Proteins - pharmacology; Bacterial Toxins - genetics; Bacteriology; Biological and medical sciences; Chimera; codons; CryIA(a) protein; CryIA(c) protein; Endotoxins; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; HELIOTHIS VIRESCENS; Hemolysin Proteins; INSECTICIDAL PROPERTIES; INSECTICIDAS; INSECTICIDE; INSECTICIDES; Larva; Lepidoptera; Lepidoptera - drug effects; MANDUCA SEXTA; Microbiology; Molecular Sequence Data; Moths - drug effects; Mutagenesis, Site-Directed; Noctuidae; NUCLEOTIDE; NUCLEOTIDES; NUCLEOTIDOS; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Plasmids; PROTEINAS UNICELULARES; PROTEINE MICROBIOLOGIQUE; Restriction Mapping; SINGLE CELL PROTEINS; Sphingidae; Heliothis virescens; Insecticide; Insecta; Bacillus thuringiensis; Molecular hybrid; Cytotoxicity; Bacillaceae; Sphingidae; Proteins; Bacillales; Gene; Structure activity relation; Arthropoda; Lepidoptera; Manduca sexta; Bacteria; Noctuidae; Invertebrata

The lepidopteran-specific, insecticidal crystal proteins of Bacillus thuringiensis vary in toxicity to different species of lepidopteran larvae. We report studies of CryIA(a) and CryIA(c), two related proteins that have different degrees of toxicity to Heliothis virescens yet very similar degrees of...