Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Evaluate the glycosaminoglycans profile and expression of heparanase in two huma...

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Published in	Anais brasileiros de dermatología Vol. 91; no. 5; pp. 595 - 600
Main Authors	Pinhal, Maria Aparecida Silva, Almeida, Maria Carolina Leal, Costa, Alessandra Scorse, Theodoro, Thérèse Rachell, Serrano, Rodrigo Lorenzetti, Machado Filho, Carlos D'Apparecida Santos
Format	Journal Article
Language	English
Published	Spain Sociedade Brasileira de Dermatologia 01.09.2016
Subjects	Carcinoma, basal cell Carcinoma, Basal Cell - enzymology Carcinoma, squamous cell Carcinoma, Squamous Cell - enzymology DERMATOLOGY Eyelids - enzymology Glucuronidase - genetics Glucuronidase - metabolism Glycosaminoglycans Glycosaminoglycans - analysis Glycosaminoglycans - metabolism Humans Hyaluronic acid Hyaluronic Acid - analysis Hyaluronic Acid - metabolism Investigation Keratinocytes - metabolism Neoplasms Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods RNA, Messenger - metabolism Skin neoplasms Skin Neoplasms - enzymology Polymerase chain reaction Carcinoma, squamous cell Glycosaminoglycans Skin neoplasms Carcinoma, basal cell Hyaluronic acid Neoplasms
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Abstract	Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
AbstractList	Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment. Background: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Objectives: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). Methods: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). Results: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. Conclusion: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment. Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer.BACKGROUND:Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer.Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control).OBJECTIVES:Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control).Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR).METHODS:Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR).The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma.RESULTS:The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma.The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.CONCLUSION:The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
Author	Theodoro, Thérèse Rachell Almeida, Maria Carolina Leal Pinhal, Maria Aparecida Silva Machado Filho, Carlos D'Apparecida Santos Costa, Alessandra Scorse Serrano, Rodrigo Lorenzetti
AuthorAffiliation	1 Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil 2 Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
AuthorAffiliation_xml	– name: 2 Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil – name: 1 Faculdade de Medicina do ABC (FMABC), Santo André, SP, Brazil – name: Faculdade de Medicina do ABC – name: Universidade Federal de São Paulo
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CitedBy_id	crossref_primary_10_3390_app10155024 crossref_primary_10_3389_fcell_2019_00068 crossref_primary_10_1080_19336918_2023_2260642 crossref_primary_10_1042_BSR20210290
Cites_doi	10.1074/jbc.M112.437236 10.1096/fj.00-0895fje 10.1074/jbc.M410412200 10.1016/S0003-2697(03)00251-3 10.1038/10525 10.2174/187152008783330824 10.1093/jnci/djh230 10.1111/j.1747-0285.2008.00741.x 10.1172/JCI13662 10.1016/j.jphotobiol.2011.10.013 10.1007/s00403-010-1117-5 10.1590/S0365-05962006000200004 10.1111/j.1538-7836.2011.04401.x 10.1172/JCI846 10.1155/2000/31748 10.1002/art.1780030306 10.1182/blood-2005-08-3301 10.1590/S0365-05962006000500003 10.1593/neo.07241 10.1016/j.jim.2007.11.014 10.1111/j.1346-8138.2011.01441.x 10.1369/0022155412459857 10.1158/1078-0432.CCR-07-1866 10.1016/j.joca.2011.12.002 10.1590/S0365-05962011000400005 10.1046/j.1087-0024.2000.00008.x 10.1136/bmj.327.7418.794 10.1007/BF00046361 10.1016/j.oraloncology.2003.08.004 10.1056/NEJM200103293441306 10.1111/j.1442-2042.2012.03086.x
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Keywords	Polymerase chain reaction Carcinoma, squamous cell Glycosaminoglycans Skin neoplasms Carcinoma, basal cell Hyaluronic acid Neoplasms
Language	English
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Snippet	Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and... Background: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell...
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SubjectTerms	Carcinoma, basal cell Carcinoma, Basal Cell - enzymology Carcinoma, squamous cell Carcinoma, Squamous Cell - enzymology DERMATOLOGY Eyelids - enzymology Glucuronidase - genetics Glucuronidase - metabolism Glycosaminoglycans Glycosaminoglycans - analysis Glycosaminoglycans - metabolism Humans Hyaluronic acid Hyaluronic Acid - analysis Hyaluronic Acid - metabolism Investigation Keratinocytes - metabolism Neoplasms Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods RNA, Messenger - metabolism Skin neoplasms Skin Neoplasms - enzymology
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Title	Expression of heparanase in basal cell carcinoma and squamous cell carcinoma
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