Structure of the Mr 140,000 Growth Hormone-Dependent Insulin-Like Growth Factor Binding Protein Complex: Determination by Reconstitution and Affinity-Labeling

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 86; no. 18; pp. 6898 - 6902
• Main Authors: Baxter, Robert C., Martin, Janet L.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.09.1989; National Acad Sciences
• Subjects: 550201 - Biochemistry- Tracer Techniques; Acromegaly - blood; Adult; AFFINITY; Affinity Labels - metabolism; Amniotic fluid; Analytical, structural and metabolic biochemistry; ANIMALS; Antiserum; AUTORADIOGRAPHY; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BIOASSAY; Biological and medical sciences; Carrier Proteins - drug effects; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; COMPLEXES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ELECTROPHORESIS; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Gels; GLYCOPROTEINS; GROWTH FACTORS; Growth Hormone - pharmacology; Growth hormones; HORMONES; Humans; IMMUNOASSAY; INSULIN; Insulin like growth factor binding proteins; Insulin-Like Growth Factor I - metabolism; Insulin-Like Growth Factor II - metabolism; INTERMEDIATE MASS NUCLEI; IODINE 125; IODINE ISOTOPES; Iodine Radioisotopes; ISOTOPES; Macromolecular Substances; MAMMALS; MAN; MITOGENS; Models, Structural; MOLECULAR STRUCTURE; MOLECULAR WEIGHT; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; Phosphates; PRIMATES; Product labeling; Protein hormones. Growth factors. Cytokines; PROTEINS; RADIOISOTOPES; Somatomedins - metabolism; VERTEBRATES; Human; Radiolabelling; Somatomedin C; Ternary complex; Gel electrophoresis; Insulin like growth factor 2; Chromatography; Autoradiography; Binding protein; Binding capacity; Reconstituted system; Serum; Models; Growth factor

To determine the structure of the high molecular weight, growth hormone-dependent complex between the insulin-like growth factors (IGF-I and IGF-II) and their binding proteins in human serum, we have reconstituted the complex from its purified component proteins and analyzed it by gel electrophoresi...