Evaluation and Minimization of Uncertainty in ITC Binding Measurements: Heat Error, Concentration Error, Saturation, and Stoichiometry

• Published in: Biochimica et biophysica acta Vol. 1861; no. 2; pp. 485 - 498
• Main Authors: Kantonen, Samuel A., Henriksen, Niel M., Gilson, Michael K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2017
• Subjects: Biophysical Phenomena - physiology; calorimetry; Calorimetry - methods; computer simulation; computer software; Data analysis; enthalpy; Error evaluation; Error minimization; experimental design; Gibbs free energy; guidelines; heat; Hot Temperature; ITC; least squares; Least-Squares Analysis; Monte Carlo Method; Protein Binding - physiology; Reproducibility of Results; Research Design; Software; stoichiometry; Thermodynamics; titration; Uncertainty; ITC; Error minimization; Data analysis; Error evaluation; MC; LS

Isothermal titration calorimetry (ITC) is uniquely useful for characterizing binding thermodynamics, because it straightforwardly provides both the binding enthalpy and free energy. However, the precision of the results depends on the experimental setup and how thermodynamic results are obtained fro...