Development of droplet digital PCR assays to quantify genes involved in nitrification and denitrification, comparison with quantitative real-time PCR and validation of assays in vineyard soil

• Published in: Canadian journal of microbiology Vol. 67; no. 2; pp. 174 - 187
• Main Authors: Voegel, Tanja M., Larrabee, Melissa M., Nelson, Louise M.
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.02.2021; Canadian Science Publishing NRC Research Press
• Subjects: Abundance; Analysis; Archaea; Archaea - genetics; Archaea - metabolism; Assaying; Bacteria; Bacteria - genetics; Bacteria - metabolism; Biochemical assays; Biogeochemical cycles; British Columbia; Canada; Carbon/nitrogen ratio; Comparative analysis; Denitrification; Denitrification - genetics; Deoxyribonucleic acid; DNA; Droplets; Expressed sequence tags; Farms; Genes; Genes, Microbial; Genetic aspects; Greenhouse gases; Growing season; Measurement; Nitrification; Nitrification - genetics; Nitrous oxide; Polymerase chain reaction; quantitative polymerase chain reaction; Real time; Real-Time Polymerase Chain Reaction - methods; Soil - chemistry; Soil bacteria; Soil Microbiology; Soil microorganisms; Soil properties; Soils; Technology application; vineyard soils; Vineyards; Canada; British Columbia; PCR numérique à gouttelettes; sol; vineyard; droplet digital PCR; dénitrification; nitrification; soil; vigne; denitrification

Quantifying genes in soil is important to relate the abundance of soil bacteria to biogeochemical cycles. Quantitative real-time PCR is widely used for quantification, but its use with environmental samples is limited by poor reaction efficiencies or by PCR inhibition through co-purified soil substa...