Transforming Growth Factor-β1 (TGF-β1)-stimulated Fibroblast to Myofibroblast Differentiation Is Mediated by Hyaluronan (HA)-facilitated Epidermal Growth Factor Receptor (EGFR) and CD44 Co-localization in Lipid Rafts

• Published in: The Journal of biological chemistry Vol. 288; no. 21; pp. 14824 - 14838
• Main Authors: Midgley, Adam C., Rogers, Mathew, Hallett, Maurice B., Clayton, Aled, Bowen, Timothy, Phillips, Aled O., Steadman, Robert
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.05.2013; American Society for Biochemistry and Molecular Biology
• Subjects: Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; CD44; Cell Differentiation - physiology; Cell Line, Transformed; Enzyme Activation - physiology; Epidermal Growth Factor Receptor (EGFR); ErbB Receptors - genetics; ErbB Receptors - metabolism; Extracellular Signal-Regulated MAP Kinases - genetics; Extracellular Signal-Regulated MAP Kinases - metabolism; Glucuronosyltransferase - genetics; Glucuronosyltransferase - metabolism; Glycobiology and Extracellular Matrices; Humans; Hyaluronan Receptors - genetics; Hyaluronan Receptors - metabolism; Hyaluronan Synthases; Hyaluronate; Hyaluronic Acid - genetics; Hyaluronic Acid - metabolism; Lipid Raft; Membrane Microdomains - genetics; Membrane Microdomains - metabolism; Myofibroblast; Myofibroblasts - cytology; Myofibroblasts - metabolism; Signal Transduction - physiology; Smad Proteins - genetics; Smad Proteins - metabolism; Transforming Growth Factor beta1 - genetics; Transforming Growth Factor beta1 - metabolism; Wound Healing - physiology; Lipid Raft; Hyaluronate; Epidermal Growth Factor Receptor (EGFR); Myofibroblast; CD44

Fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine transforming growth factor-β1 (TGF-β1). Myofibroblasts express α-smooth muscle actin and are present in granulation tissue, where they are responsible for wound contraction. Our previo...