High-yield isolation of murine microglia by mild trypsinization

• Published in: Glia Vol. 44; no. 3; pp. 183 - 189
• Main Authors: Saura, Josep, Tusell, Josep Maria, Serratosa, Joan
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.12.2003; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Brain - cytology; CD11b; Cell Count; Cell Division; Cell Separation - methods; Cells, Cultured; Fundamental and applied biological sciences. Psychology; in vitro; interferon γ; Isolated neuron and nerve. Neuroglia; lipopolysaccharide; M-CSF; Mice; Mice, Inbred C57BL; Microglia - cytology; Microglia - metabolism; NF-kappa B - metabolism; Nitric Oxide - metabolism; Phagocytosis; trypsin; Trypsin - pharmacology; Tumor Necrosis Factor-alpha - metabolism; Vertebrates: nervous system and sense organs; M-CSF; Neuroglia; Rodentia; Central nervous system; In vitro; Encephalon; Microglia; CDllb; Vertebrata; Mammalia; Mouse; interferon γ; Lipopolysaccharide; trypsin; Technique; Gamma interferon

Microglia can be isolated with high purity but low yield by shaking off loosely adherent cells from mixed glial cultures. Here we describe a new technique for isolating microglia with an average yield close to 2,000,000 microglial cells/mouse pup, more than five times higher than that of the shaking...