Towards a standardized human proteome database: Quantitative proteome profiling of living cells

• Published in: Proteomics (Weinheim) Vol. 4; no. 5; pp. 1314 - 1323
• Main Authors: Traxler, Elisabeth, Bayer, Editha, Stöckl, Johannes, Mohr, Thomas, Lenz, Christof, Gerner, Christopher
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.05.2004; WILEY‐VCH Verlag
• Subjects: Autoradiography; Cells, Cultured; Databases, Factual - standards; Electrophoresis, Gel, Two-Dimensional; Endothelial cells; Endothelium, Vascular - chemistry; Endothelium, Vascular - drug effects; Fluorescence; Fluorescence detection; Humans; Jurkat Cells; Kinetics; Leukaemia; Lymphocyte Activation; Peptide Fragments - analysis; Phytohemagglutinins - pharmacology; Proteins - chemistry; Proteins - metabolism; Proteome - analysis; Proteome - chemistry; Proteome - metabolism; Sensitivity and Specificity; Subcellular Fractions; Sulfur Radioisotopes - metabolism; T-Lymphocytes - chemistry; T-Lymphocytes - drug effects; Trypsin; Two-dimensional gel electrophoresis; Umbilical Veins - cytology; Vascular Endothelial Growth Factor A - drug effects

Comparative proteome profiling, performed by two‐dimensional polyacrylamide gel electrophoresis or multidimensional protein identification technology, usually relies on the relative comparison of samples of interest with respect to a reference. Currently, no standardized quantitative protein express...