Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis

• Published in: Angewandte Chemie International Edition Vol. 59; no. 40; pp. 17403 - 17407
• Main Authors: Kawaguchi, Daisuke, Kodama, Ayumi, Abe, Naoko, Takebuchi, Kei, Hashiya, Fumitaka, Tomoike, Fumiaki, Nakamoto, Kosuke, Kimura, Yasuaki, Shimizu, Yoshihiro, Abe, Hiroshi
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 28.09.2020
• Edition: International ed. in English
• Subjects: backbone modification; Complex formation; E coli; Epidermal Growth Factor - genetics; Epidermal Growth Factor - metabolism; Escherichia coli - metabolism; mRNA; mRNA turnover; Peptide Chain Initiation, Translational; Phosphorothioate; phosphorothioate oligonucleotides; Phosphorothioate Oligonucleotides - chemistry; Protein Biosynthesis; protein expression; Protein folding; Protein synthesis; Proteins; Ribosomes; Ribosomes - metabolism; RNA; RNA modification; RNA, Messenger - chemistry; RNA, Messenger - metabolism; Transcription; Translation; Translation initiation; mRNA; phosphorothioate oligonucleotides; RNA; protein expression; backbone modification

Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15...