Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis

Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15...

Full description

Saved in:
Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 59; no. 40; pp. 17403 - 17407
Main Authors Kawaguchi, Daisuke, Kodama, Ayumi, Abe, Naoko, Takebuchi, Kei, Hashiya, Fumitaka, Tomoike, Fumiaki, Nakamoto, Kosuke, Kimura, Yasuaki, Shimizu, Yoshihiro, Abe, Hiroshi
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 28.09.2020
EditionInternational ed. in English
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22‐fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5′‐end to the initiation codon. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non‐natural mRNA. Phosphorothioate (PS) modification of mRNA leads to enhanced protein expression in a reconstituted E. coli translation system. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that translation initiation is more efficient on the modified mRNA.
AbstractList Abstract Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22‐fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5′‐end to the initiation codon. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non‐natural mRNA.
Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22‐fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5′‐end to the initiation codon. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non‐natural mRNA.
Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell‐free translation system. Protein synthesis from PS‐mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22‐fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5′‐end to the initiation codon. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non‐natural mRNA. Phosphorothioate (PS) modification of mRNA leads to enhanced protein expression in a reconstituted E. coli translation system. Single‐turnover analysis of PS‐mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that translation initiation is more efficient on the modified mRNA.
Author Kodama, Ayumi
Shimizu, Yoshihiro
Kimura, Yasuaki
Kawaguchi, Daisuke
Tomoike, Fumiaki
Hashiya, Fumitaka
Nakamoto, Kosuke
Abe, Naoko
Takebuchi, Kei
Abe, Hiroshi
Author_xml – sequence: 1
  givenname: Daisuke
  surname: Kawaguchi
  fullname: Kawaguchi, Daisuke
  organization: Nagoya University
– sequence: 2
  givenname: Ayumi
  surname: Kodama
  fullname: Kodama, Ayumi
  organization: Nagoya University
– sequence: 3
  givenname: Naoko
  orcidid: 0000-0001-6062-3515
  surname: Abe
  fullname: Abe, Naoko
  organization: Nagoya University
– sequence: 4
  givenname: Kei
  surname: Takebuchi
  fullname: Takebuchi, Kei
  organization: Nagoya University
– sequence: 5
  givenname: Fumitaka
  orcidid: 0000-0003-1546-4126
  surname: Hashiya
  fullname: Hashiya, Fumitaka
  organization: Nagoya University
– sequence: 6
  givenname: Fumiaki
  orcidid: 0000-0003-2724-2272
  surname: Tomoike
  fullname: Tomoike, Fumiaki
  organization: Nagoya University
– sequence: 7
  givenname: Kosuke
  orcidid: 0000-0001-7813-6555
  surname: Nakamoto
  fullname: Nakamoto, Kosuke
  organization: Nagoya University
– sequence: 8
  givenname: Yasuaki
  orcidid: 0000-0002-3609-602X
  surname: Kimura
  fullname: Kimura, Yasuaki
  organization: Nagoya University
– sequence: 9
  givenname: Yoshihiro
  orcidid: 0000-0003-3499-1394
  surname: Shimizu
  fullname: Shimizu, Yoshihiro
  email: yshimizu@riken.jp
  organization: RIKEN
– sequence: 10
  givenname: Hiroshi
  orcidid: 0000-0003-0048-3789
  surname: Abe
  fullname: Abe, Hiroshi
  email: h-abe@chem.nagoya-u.ac.jp
  organization: Tokai National Higher Education and Research System
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32627275$$D View this record in MEDLINE/PubMed
BookMark eNqFkT1v2zAQhokgRWInWTMGBLp0kcsvifJoGG5jwE2DNJkFSjrBDGTSJekW3vrTc4LSBOjS6Q68hw8_3ik5dd4BIdeczThj4rNxFmaCCcY05_yETHgueCa1lqfYKykzXeb8nExjfEa-LFlxRs6lKIQWOp-QP_dbH_dbH3zaWm8S0G--tZ1tTLLeUd_R3cPdgi6aBnoIOI80bYE-DCQOH4NxsR_ZtbPJjm3y9D74X7YddAHoqkOjBZeG5QTW0R9Hh55o4yX50Jk-wtVrvSBPX1aPy9ts8_3rernYZE3OC56BLESta6HEXORFrdvCNLxRrJQKsBjdGKHyea10beasxu8A1hpdKI5k2Up5QT6N3n3wPw8QU7WzER_VGwf-ECs0s0LiKTmiH_9Bn_0hOLwdUkqJkivBkZqNVBN8jAG6ah_szoRjxVk1ZFMN2VRv2eCGm1ftod5B-4b_DQOB-Qj8tj0c_6OrFnfr1bv8BYK8nTA
CitedBy_id crossref_primary_10_1021_acschembio_0c00864
crossref_primary_10_1021_acs_jpcb_0c10192
crossref_primary_10_1002_ddr_22164
crossref_primary_10_1021_acschembio_2c00199
crossref_primary_10_1016_j_coi_2022_102249
crossref_primary_10_1261_rna_077099_120
crossref_primary_10_1039_D2CB00116K
crossref_primary_10_1002_anie_202100352
crossref_primary_10_1002_chem_202201115
crossref_primary_10_1021_acschembio_1c00569
crossref_primary_10_1261_rna_079396_122
crossref_primary_10_1021_acsomega_3c08635
crossref_primary_10_1016_j_cbpa_2024_102479
crossref_primary_10_3390_pharmaceutics13060800
crossref_primary_10_1021_acs_accounts_1c00550
crossref_primary_10_1146_annurev_biophys_111622_091203
crossref_primary_10_1021_acs_molpharmaceut_3c00803
crossref_primary_10_1039_D1SC01194D
crossref_primary_10_1039_D1BM01658J
crossref_primary_10_3389_fcell_2022_901510
crossref_primary_10_1016_j_ejpb_2024_114234
crossref_primary_10_1248_cpb_c21_00960
crossref_primary_10_1002_ange_202100352
crossref_primary_10_1016_j_addr_2023_114972
crossref_primary_10_1021_acschembio_1c00996
Cites_doi 10.1111/j.1432-1033.1970.tb00961.x
10.1039/c1cs15102a
10.1021/ol802910k
10.1016/j.gene.2005.06.037
10.1146/annurev-pharmtox-010818-021050
10.1016/j.ymthe.2019.02.012
10.1021/jo301052v
10.1038/nbt.3948
10.1074/jbc.REV118.002814
10.1016/S0968-0004(97)01052-9
10.1093/nar/19.3.547
10.1146/annurev.bi.54.070185.002055
10.1016/0168-1656(94)90166-X
10.1128/MMBR.69.1.101-123.2005
10.15252/embr.201642943
10.1002/cbic.201500046
10.1093/nar/15.10.4145
10.1021/cb8002946
10.1038/90802
10.1007/978-1-62703-782-2_19
10.1371/journal.pgen.1005613
10.1126/science.aau3369
10.1093/emboj/cdg525
10.1039/C6CC08473G
10.3390/pharmaceutics7030137
10.1073/pnas.1721431115
10.1016/j.ymthe.2018.11.018
10.1089/nat.2014.0506
10.1021/acs.bioconjchem.6b00090
10.1038/nrd.2017.243
10.1074/jbc.M205405200
10.1073/pnas.87.19.7668
10.1038/nbt.3765
ContentType Journal Article
Copyright 2020 Wiley‐VCH GmbH
2020 Wiley-VCH GmbH.
Copyright_xml – notice: 2020 Wiley‐VCH GmbH
– notice: 2020 Wiley-VCH GmbH.
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
K9.
7X8
DOI 10.1002/anie.202007111
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Nucleic Acids Abstracts
ProQuest Health & Medical Complete (Alumni)
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
ProQuest Health & Medical Complete (Alumni)
Nucleic Acids Abstracts
MEDLINE - Academic
DatabaseTitleList CrossRef
ProQuest Health & Medical Complete (Alumni)
MEDLINE

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1521-3773
Edition International ed. in English
EndPage 17407
ExternalDocumentID 10_1002_anie_202007111
32627275
ANIE202007111
Genre shortCommunication
Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: Japan Society for the Promotion of Science
  funderid: 16H04178; 18K14357
– fundername: Japan Science and Technology Agency
  funderid: JPMJCR18S1
GroupedDBID ---
-DZ
-~X
.3N
.GA
05W
0R~
10A
1L6
1OB
1OC
1ZS
23M
33P
3SF
3WU
4.4
4ZD
50Y
50Z
51W
51X
52M
52N
52O
52P
52S
52T
52U
52W
52X
53G
5GY
5RE
5VS
66C
6TJ
702
7PT
8-0
8-1
8-3
8-4
8-5
8UM
930
A03
AAESR
AAEVG
AAHHS
AANLZ
AAONW
AASGY
AAXRX
AAZKR
ABCQN
ABCUV
ABEML
ABIJN
ABLJU
ABPPZ
ABPVW
ACAHQ
ACCFJ
ACCZN
ACFBH
ACGFS
ACIWK
ACNCT
ACPOU
ACPRK
ACSCC
ACXBN
ACXQS
ADBBV
ADEOM
ADIZJ
ADKYN
ADMGS
ADOZA
ADXAS
ADZMN
ADZOD
AEEZP
AEIGN
AEIMD
AEQDE
AEUQT
AEUYR
AFBPY
AFFNX
AFFPM
AFGKR
AFPWT
AFRAH
AFZJQ
AHBTC
AHMBA
AITYG
AIURR
AIWBW
AJBDE
AJXKR
ALAGY
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMBMR
AMYDB
ATUGU
AUFTA
AZBYB
AZVAB
B-7
BAFTC
BDRZF
BFHJK
BHBCM
BMNLL
BMXJE
BNHUX
BROTX
BRXPI
BTSUX
BY8
CS3
D-E
D-F
D0L
DCZOG
DPXWK
DR1
DR2
DRFUL
DRSTM
EBS
F00
F01
F04
F5P
G-S
G.N
GNP
GODZA
H.T
H.X
HBH
HGLYW
HHY
HHZ
HZ~
IX1
J0M
JPC
KQQ
LATKE
LAW
LC2
LC3
LEEKS
LH4
LITHE
LOXES
LP6
LP7
LUTES
LYRES
M53
MEWTI
MK4
MRFUL
MRSTM
MSFUL
MSSTM
MXFUL
MXSTM
N04
N05
N9A
NF~
NNB
O66
O9-
OIG
P2P
P2W
P2X
P4D
PQQKQ
Q.N
Q11
QB0
QRW
R.K
RNS
ROL
RWI
RX1
RYL
SUPJJ
TN5
UB1
UPT
UQL
V2E
VQA
W8V
W99
WBFHL
WBKPD
WH7
WIB
WIH
WIK
WJL
WOHZO
WQJ
WRC
WXSBR
WYISQ
XG1
XPP
XSW
XV2
YZZ
ZZTAW
~IA
~KM
~WT
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7TM
K9.
7X8
ID FETCH-LOGICAL-c5161-e362b7b2429256b7d6ac1c40834ec40a7ca2459b47ba90b007e0da7641d6a8d33
IEDL.DBID DR2
ISSN 1433-7851
IngestDate Fri Aug 16 05:05:07 EDT 2024
Thu Oct 10 18:22:48 EDT 2024
Fri Aug 23 00:49:04 EDT 2024
Sat Sep 28 08:23:47 EDT 2024
Sat Aug 24 01:05:00 EDT 2024
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 40
Keywords mRNA
phosphorothioate oligonucleotides
RNA
protein expression
backbone modification
Language English
License 2020 Wiley-VCH GmbH.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c5161-e362b7b2429256b7d6ac1c40834ec40a7ca2459b47ba90b007e0da7641d6a8d33
Notes These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-7813-6555
0000-0003-3499-1394
0000-0001-6062-3515
0000-0003-1546-4126
0000-0002-3609-602X
0000-0003-2724-2272
0000-0003-0048-3789
OpenAccessLink https://rss.onlinelibrary.wiley.com/doi/am-pdf/10.1002/anie.202007111
PMID 32627275
PQID 2444281421
PQPubID 946352
PageCount 5
ParticipantIDs proquest_miscellaneous_2420633625
proquest_journals_2444281421
crossref_primary_10_1002_anie_202007111
pubmed_primary_32627275
wiley_primary_10_1002_anie_202007111_ANIE202007111
PublicationCentury 2000
PublicationDate September 28, 2020
PublicationDateYYYYMMDD 2020-09-28
PublicationDate_xml – month: 09
  year: 2020
  text: September 28, 2020
  day: 28
PublicationDecade 2020
PublicationPlace Germany
PublicationPlace_xml – name: Germany
– name: Weinheim
PublicationTitle Angewandte Chemie International Edition
PublicationTitleAlternate Angew Chem Int Ed Engl
PublicationYear 2020
Publisher Wiley Subscription Services, Inc
Publisher_xml – name: Wiley Subscription Services, Inc
References 2018; 361
1991; 19
2015; 16
1997; 22
2011; 40
2019; 59
2015; 11
2002; 277
2014; 24
2016; 17
2015; 7
2012; 77
1987; 15
1970; 13
2005; 69
2009; 11
2017; 53
2018; 17
1990; 87
2005; 361
2017; 35
2018; 115
1994; 34
2001; 19
2019; 27
2014
2009; 4
2019; 294
2016; 27
1985; 54
2003; 22
e_1_2_2_3_2
e_1_2_2_24_2
e_1_2_2_4_2
e_1_2_2_5_1
e_1_2_2_22_2
e_1_2_2_23_1
e_1_2_2_6_2
e_1_2_2_21_2
e_1_2_2_20_2
e_1_2_2_1_1
e_1_2_2_2_2
e_1_2_2_40_1
e_1_2_2_41_1
e_1_2_2_42_2
e_1_2_2_43_1
e_1_2_2_7_2
e_1_2_2_28_2
e_1_2_2_29_1
e_1_2_2_8_1
e_1_2_2_27_2
e_1_2_2_44_2
e_1_2_2_45_2
e_1_2_2_9_2
e_1_2_2_26_1
e_1_2_2_13_2
e_1_2_2_14_1
Shimizu Y. (e_1_2_2_25_2) 2014
e_1_2_2_36_2
e_1_2_2_37_1
e_1_2_2_12_2
e_1_2_2_11_2
e_1_2_2_38_2
e_1_2_2_10_2
e_1_2_2_39_2
e_1_2_2_19_2
e_1_2_2_30_2
e_1_2_2_18_2
e_1_2_2_31_2
e_1_2_2_32_1
e_1_2_2_33_1
e_1_2_2_16_2
e_1_2_2_17_1
e_1_2_2_15_2
e_1_2_2_34_2
e_1_2_2_35_2
References_xml – volume: 27
  start-page: 785
  year: 2019
  end-page: 793
  publication-title: Mol. Ther.
– volume: 11
  start-page: 967
  year: 2009
  end-page: 970
  publication-title: Org. Lett.
– volume: 87
  start-page: 7668
  year: 1990
  end-page: 7672
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 59
  start-page: 605
  year: 2019
  end-page: 630
  publication-title: Annu. Rev. Pharmacol. Toxicol.
– volume: 361
  start-page: 13
  year: 2005
  end-page: 37
  publication-title: Gene
– volume: 27
  start-page: 849
  year: 2016
  end-page: 853
  publication-title: Bioconjugate Chem.
– volume: 19
  start-page: 547
  year: 1991
  end-page: 552
  publication-title: Nucleic Acids Res.
– volume: 54
  start-page: 367
  year: 1985
  end-page: 402
  publication-title: Annu. Rev. Biochem.
– volume: 15
  start-page: 4145
  year: 1987
  end-page: 4162
  publication-title: Nucleic Acids Res.
– volume: 53
  start-page: 541
  year: 2017
  end-page: 544
  publication-title: Chem. Commun.
– volume: 13
  start-page: 558
  year: 1970
  end-page: 564
  publication-title: Eur. J. Biochem.
– volume: 7
  start-page: 137
  year: 2015
  end-page: 151
  publication-title: Pharmaceutics
– volume: 69
  start-page: 101
  year: 2005
  end-page: 123
  publication-title: Microbiol. Mol. Biol. Rev.
– volume: 4
  start-page: 93
  year: 2009
  end-page: 107
  publication-title: ACS Chem. Biol.
– volume: 27
  start-page: 710
  year: 2019
  end-page: 728
  publication-title: Mol. Ther.
– volume: 11
  year: 2015
  publication-title: PLoS Genet.
– volume: 40
  start-page: 5829
  year: 2011
  end-page: 5843
  publication-title: Chem. Soc. Rev.
– volume: 19
  start-page: 751
  year: 2001
  end-page: 755
  publication-title: Nat. Biotechnol.
– volume: 22
  start-page: 211
  year: 1997
  end-page: 216
  publication-title: Trends Biochem. Sci.
– volume: 115
  start-page: E6731
  year: 2018
  end-page: E6740
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 361
  start-page: 1234
  year: 2018
  end-page: 1238
  publication-title: Science
– volume: 277
  start-page: 33825
  year: 2002
  end-page: 33832
  publication-title: J. Biol. Chem.
– volume: 16
  start-page: 1109
  year: 2015
  end-page: 1114
  publication-title: ChemBioChem
– volume: 22
  start-page: 5593
  year: 2003
  end-page: 5601
  publication-title: EMBO J.
– volume: 35
  start-page: 238
  year: 2017
  end-page: 248
  publication-title: Nat. Biotechnol.
– volume: 17
  start-page: 1776
  year: 2016
  end-page: 1784
  publication-title: EMBO Rep.
– volume: 77
  start-page: 7913
  year: 2012
  end-page: 7922
  publication-title: J. Org. Chem.
– volume: 294
  start-page: 2076
  year: 2019
  end-page: 2084
  publication-title: J. Biol. Chem.
– volume: 35
  start-page: 845
  year: 2017
  end-page: 851
  publication-title: Nat. Biotechnol.
– start-page: 275
  year: 2014
  end-page: 284
– volume: 34
  start-page: 61
  year: 1994
  end-page: 69
  publication-title: J. Biotechnol.
– volume: 24
  start-page: 374
  year: 2014
  end-page: 387
  publication-title: Nucleic Acid Ther.
– volume: 17
  start-page: 261
  year: 2018
  end-page: 279
  publication-title: Nat. Rev. Drug Discovery
– ident: e_1_2_2_18_2
  doi: 10.1111/j.1432-1033.1970.tb00961.x
– ident: e_1_2_2_43_1
– ident: e_1_2_2_9_2
  doi: 10.1039/c1cs15102a
– ident: e_1_2_2_1_1
– ident: e_1_2_2_38_2
  doi: 10.1021/ol802910k
– ident: e_1_2_2_36_2
  doi: 10.1016/j.gene.2005.06.037
– ident: e_1_2_2_13_2
  doi: 10.1146/annurev-pharmtox-010818-021050
– ident: e_1_2_2_37_1
– ident: e_1_2_2_2_2
  doi: 10.1016/j.ymthe.2019.02.012
– ident: e_1_2_2_23_1
– ident: e_1_2_2_39_2
  doi: 10.1021/jo301052v
– ident: e_1_2_2_11_2
  doi: 10.1038/nbt.3948
– ident: e_1_2_2_28_2
  doi: 10.1074/jbc.REV118.002814
– ident: e_1_2_2_42_2
  doi: 10.1016/S0968-0004(97)01052-9
– ident: e_1_2_2_21_2
  doi: 10.1093/nar/19.3.547
– ident: e_1_2_2_19_2
  doi: 10.1146/annurev.bi.54.070185.002055
– ident: e_1_2_2_29_1
– ident: e_1_2_2_22_2
  doi: 10.1016/0168-1656(94)90166-X
– ident: e_1_2_2_27_2
  doi: 10.1128/MMBR.69.1.101-123.2005
– ident: e_1_2_2_26_1
– ident: e_1_2_2_31_2
  doi: 10.15252/embr.201642943
– ident: e_1_2_2_40_1
  doi: 10.1002/cbic.201500046
– ident: e_1_2_2_20_2
  doi: 10.1093/nar/15.10.4145
– ident: e_1_2_2_45_2
  doi: 10.1021/cb8002946
– ident: e_1_2_2_24_2
  doi: 10.1038/90802
– start-page: 275
  volume-title: Cell-Free Protein Synthesis: Methods and Protocols, Vol. 1118
  year: 2014
  ident: e_1_2_2_25_2
  doi: 10.1007/978-1-62703-782-2_19
  contributor:
    fullname: Shimizu Y.
– ident: e_1_2_2_34_2
  doi: 10.1371/journal.pgen.1005613
– ident: e_1_2_2_15_2
  doi: 10.1126/science.aau3369
– ident: e_1_2_2_44_2
  doi: 10.1093/emboj/cdg525
– ident: e_1_2_2_16_2
  doi: 10.1039/C6CC08473G
– ident: e_1_2_2_33_1
– ident: e_1_2_2_17_1
– ident: e_1_2_2_14_1
– ident: e_1_2_2_7_2
  doi: 10.3390/pharmaceutics7030137
– ident: e_1_2_2_8_1
– ident: e_1_2_2_32_1
  doi: 10.1073/pnas.1721431115
– ident: e_1_2_2_4_2
  doi: 10.1016/j.ymthe.2018.11.018
– ident: e_1_2_2_41_1
– ident: e_1_2_2_5_1
– ident: e_1_2_2_10_2
  doi: 10.1089/nat.2014.0506
– ident: e_1_2_2_6_2
  doi: 10.1021/acs.bioconjchem.6b00090
– ident: e_1_2_2_3_2
  doi: 10.1038/nrd.2017.243
– ident: e_1_2_2_30_2
  doi: 10.1074/jbc.M205405200
– ident: e_1_2_2_35_2
  doi: 10.1073/pnas.87.19.7668
– ident: e_1_2_2_12_2
  doi: 10.1038/nbt.3765
SSID ssj0028806
Score 2.5234332
Snippet Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared,...
Messenger RNAs (mRNAs) with phosphorothioate modification (PS-mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared,...
Abstract Messenger RNAs (mRNAs) with phosphorothioate modification (PS‐mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were...
SourceID proquest
crossref
pubmed
wiley
SourceType Aggregation Database
Index Database
Publisher
StartPage 17403
SubjectTerms backbone modification
Complex formation
E coli
Epidermal Growth Factor - genetics
Epidermal Growth Factor - metabolism
Escherichia coli - metabolism
mRNA
mRNA turnover
Peptide Chain Initiation, Translational
Phosphorothioate
phosphorothioate oligonucleotides
Phosphorothioate Oligonucleotides - chemistry
Protein Biosynthesis
protein expression
Protein folding
Protein synthesis
Proteins
Ribosomes
Ribosomes - metabolism
RNA
RNA modification
RNA, Messenger - chemistry
RNA, Messenger - metabolism
Transcription
Translation
Translation initiation
Title Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fanie.202007111
https://www.ncbi.nlm.nih.gov/pubmed/32627275
https://www.proquest.com/docview/2444281421
https://search.proquest.com/docview/2420633625
Volume 59
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3JTsMwELUQF7iwL2WTkZA4pU0cZ-mxglaARIUKSL1F3hAIkVRNe4ATn86M3QQKByQ4ZbGTOB5P5tmZeUPICQNlNn7cxoge6fFUB57gD8zTIedM60THlqz6uh9f3POrYTT8EsXv-CHqBTfUDPu9RgUXsmx9koZiBDbM73CtLbDBvUGYoE_X-aDmj2IwOF14URh6mIW-Ym30WWv-8nmr9ANqziNXa3p6q0RUjXYeJ8_N6UQ21ds3Psf_vNUaWZnhUtpxA2mdLJh8gyydVengNsn7zWNRjh6LMYj2qQCESq8LjX5GVrS0eKAvg36HdpQCQ4b8EyUFbEkHWBMKrU10fnf0Eh2W3O6koDcuFhBuNza0axktwBDiaUzESW9fc7hP-VRukfte9-7swpulb_BUBDjSM2AbZSIZJsSKYgliFypQHDAfN7ARiRKMR23JEynaPqh_YnwtkpgHUDPVYbhNFvMiN7uEapYC7FBK-wY-OlyLiKu2UBIm90rHqd8gp5X4spFj6cgcHzPLsEezukcb5KCSbjbT1jIDiAOzsIAzKD6ui6F_8eeJyE0xxToM0By8UtQgO25U1I8CCMwAB0IJs7L9pQ1Zp3_ZrY_2_nLRPlnGfXRcYekBWZyMp-YQ0NFEHlkN-AAtFwaj
link.rule.ids 315,783,787,1378,27938,27939,46308,46732
linkProvider Wiley-Blackwell
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3JTsMwELUQHMqFfSmrkZA4BRLHWXqsSlELtEIFJG6RtwqESFDTHuDEpzMTN0GFAxKcstjZPB7PszPzhpBjBsps3LCBET3S4bH2HMGHzNE-50zrSIcFWXWvH3bu-eVDUHoTYiyM5YeoFtxQM4rxGhUcF6TPvlhDMQQbJni42OZhdO8C6LyPSQzOBxWDFIPuaQOMfN_BPPQlb6PLzmavn7VLP8DmLHYtjM_FMpHla1ufk-fTyVieqvdvjI7_-q4VsjSFprRp-9IqmTPpGqm1yoxw6-Tj5jHLXx-zEUj3KQOQSnuZRlejQro0G9KXQb9Jm0qBLUMKipwCvKQDrAmFhVm0rne0iz5Ldnec0RsbDgi3GxnaLkgtwBbiaczFSW_fUrhP_pRvkPuL9l2r40wzODgqACjpGDCPMpIMc2IFoQTJC-UpDrCPG9iISAnGg4bkkRQNF0aAyLhaRCH3oGasfX-TzKdZarYJ1SwG5KGUdg2MO1yLgKuGUBLm90qHsVsnJ6X8kldL1JFYSmaWYIsmVYvWyV4p3mSqsHkCKAcmYh5nUHxUFUP74v8TkZpsgnUYADr4pKBOtmy3qB4FKJgBFIQSVgj3l3dImv1uuzra-ctFh6TWuetdJ9fd_tUuWcTz6MfC4j0yPx5NzD6ApbE8KNThE5IlCr0
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT9tAEF4hkIAL9AUEaNlKlXpyYq_XjxwjSESgsaJAJG7WviJQVTuKnQOc-OnMeGO3aQ9I5eTHru3dnR3Pt_bMN4R8Y6DMxg27GNEjHR5rzxF8xhztc860jnRYkVWPkvByyq_ugrs_ovgtP0TzwQ01o3pfo4LP9azzmzQUI7BhfYff2jwM7t3ioe-iU9fFpCGQYjA7bXyR7zuYhr6mbXRZZ_36dbP0D9Zch66V7RnsE1G32rqc_GwvS9lWT38ROr6lW-_I3gqY0p6dSe_Jhsk-kJ3zOh_cR_I8vs-L-X2-ANk-5ABR6SjX6GhUyZbmM_prkvRoTymwZEhAUVAAl3SCNaGwMorW8Y4O0WPJ7pY5HdtgQLjdwtB-RWkBlhBPYyZOevOYwX2Kh-ITmQ76t-eXzip_g6MCAJKOAeMoI8kwI1YQSpC7UJ7iAPq4gY2IlGA86EoeSdF1Qf8j42oRhdyDmrH2_QOymeWZOSJUsxhwh1LaNfDW4VoEXHWFkrC6VzqM3Rb5XosvnVuajtQSMrMURzRtRrRFTmvppit1LVLAOLAM8ziD4q9NMYwv_j0RmcmXWIcBnIMuBS1yaGdF8yjAwAyAIJSwSravtCHtJcN-c3T8Pxedke3xxSD9MUyuT8gunkYnFhafks1ysTSfASmV8kulDC9kywls
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Phosphorothioate+Modification+of+mRNA+Accelerates+the+Rate+of+Translation+Initiation+to+Provide+More+Efficient+Protein+Synthesis&rft.jtitle=Angewandte+Chemie+International+Edition&rft.au=Kawaguchi%2C+Daisuke&rft.au=Kodama%2C+Ayumi&rft.au=Abe%2C+Naoko&rft.au=Takebuchi%2C+Kei&rft.date=2020-09-28&rft.eissn=1521-3773&rft.volume=59&rft.issue=40&rft.spage=17403&rft.epage=17407&rft_id=info:doi/10.1002%2Fanie.202007111&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1433-7851&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1433-7851&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1433-7851&client=summon