Separation of five compounds from leaves of Andrographis paniculata (Burm. f.) Nees by off-line two-dimensional high-speed counter-current chromatography combined with gradient and recycling elution

• Published in: Journal of separation science Vol. 38; no. 9; pp. 1476 - 1483
• Main Authors: Zhang, Li, Liu, Qi, Yu, Jingang, Zeng, Hualiang, Jiang, Shujing, Chen, Xiaoqing
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.05.2015; Wiley Subscription Services, Inc
• Subjects: Andrographis - chemistry; Andrographis paniculata (Burm. f.) Nees; Chromatography; Chromatography, High Pressure Liquid; Diterpenes - chemistry; Diterpenes - isolation & purification; Elution; Flavones - chemistry; Flavones - isolation & purification; Flavonoids - chemistry; Flavonoids - isolation & purification; Flowers & plants; Glucosides - chemistry; Glucosides - isolation & purification; Glucuronates - chemistry; Glucuronates - isolation & purification; Gradient elution; High speed; High-speed counter-current chromatography; Metabolites; Methyl alcohol; Molecular Conformation; Natural products; NMR spectroscopy; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Leaves - chemistry; Recycling; Recycling elution; Separation; Two dimensional; Recycling elution; Andrographis paniculata (Burm. f.) Nees; Gradient elution; High-speed counter-current chromatography

An off‐line two‐dimensional high‐speed counter‐current chromatography method combined with gradient and recycling elution mode was established to isolate terpenoids and flavones from the leaves of Andrographis paniculata (Burm. f.) Nees. By using the solvent systems composed of n‐hexane/ethyl acetat...