Bacterial growth monitoring in a microfluidic device by confocal reflection microscopy

• Published in: Journal of bioscience and bioengineering Vol. 110; no. 1; pp. 130 - 133
• Main Authors: Yawata, Yutaka, Toda, Kensuke, Setoyama, Erika, Fukuda, Junji, Suzuki, Hiroaki, Uchiyama, Hiroo, Nomura, Nobuhiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2010; Elsevier
• Subjects: Bacteria; Bacteriological Techniques - instrumentation; Bacteriological Techniques - methods; Bio-chip; Biological and medical sciences; Biotechnology; Confocal microscopy; DNA; Fundamental and applied biological sciences. Psychology; Imaging, Three-Dimensional - methods; MEMS; Microfludic device; Microfluidic Analytical Techniques; Microscopy, Confocal; Pseudomonas aeruginosa; Pseudomonas aeruginosa - growth & development; μTAS; Microfludic device; μTAS; Pseudomonas aeruginosa; Confocal microscopy; MEMS; Bio-chip; Pseudomonadales; Growth; Microequipment; Pseudomonadaceae; Bacteria; Microelectromechanical device; Monitoring

The feasibility of a method to nondestructively measure planktonic bacterial growth in a microfluidic device was addressed. Here, we report that the growth of Pseudomonas aeruginosa in a microfluidic device could be measured by a three-dimensional image analysis technique based on confocal reflectio...