Bacterial growth monitoring in a microfluidic device by confocal reflection microscopy

The feasibility of a method to nondestructively measure planktonic bacterial growth in a microfluidic device was addressed. Here, we report that the growth of Pseudomonas aeruginosa in a microfluidic device could be measured by a three-dimensional image analysis technique based on confocal reflectio...

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Bibliographic Details
Published inJournal of bioscience and bioengineering Vol. 110; no. 1; pp. 130 - 133
Main Authors Yawata, Yutaka, Toda, Kensuke, Setoyama, Erika, Fukuda, Junji, Suzuki, Hiroaki, Uchiyama, Hiroo, Nomura, Nobuhiko
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.07.2010
Elsevier
Subjects
Bacteria
Bacteriological Techniques - instrumentation
Bacteriological Techniques - methods
Bio-chip
Biological and medical sciences
Biotechnology
Confocal microscopy
DNA
Fundamental and applied biological sciences. Psychology
Imaging, Three-Dimensional - methods
MEMS
Microfludic device
Microfluidic Analytical Techniques
Microscopy, Confocal
Pseudomonas aeruginosa
Pseudomonas aeruginosa - growth & development
μTAS
Microfludic device
μTAS
Pseudomonas aeruginosa
Confocal microscopy
MEMS
Bio-chip
Pseudomonadales
Growth
Microequipment
Pseudomonadaceae
Bacteria
Microelectromechanical device
Monitoring
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Summary:The feasibility of a method to nondestructively measure planktonic bacterial growth in a microfluidic device was addressed. Here, we report that the growth of Pseudomonas aeruginosa in a microfluidic device could be measured by a three-dimensional image analysis technique based on confocal reflection microscopy in a time-course.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2010.01.009