Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody

• Published in: Science (American Association for the Advancement of Science) Vol. 371; no. 6531; pp. 823 - 829
• Main Authors: Rappazzo, C. Garrett, Tse, Longping V., Kaku, Chengzi I., Wrapp, Daniel, Sakharkar, Mrunal, Huang, Deli, Deveau, Laura M., Yockachonis, Thomas J., Herbert, Andrew S., Battles, Michael B., O’Brien, Cecilia M., Brown, Michael E., Geoghegan, James C., Belk, Jonathan, Peng, Linghang, Yang, Linlin, Hou, Yixuan, Scobey, Trevor D., Burton, Dennis R., Nemazee, David, Dye, John M., Voss, James E., Gunn, Bronwyn M., McLellan, Jason S., Baric, Ralph S., Gralinski, Lisa E., Walker, Laura M.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 19.02.2021; American Association for the Advancement of Science
• Subjects: Affinity; Angiotensin-Converting Enzyme 2 - metabolism; Animal models; Animals; Antibodies; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - metabolism; Antibodies, Viral - genetics; Antibodies, Viral - immunology; Antibodies, Viral - metabolism; Antibody Affinity; Betacoronavirus - immunology; Binding Sites; Binding Sites, Antibody; Broadly Neutralizing Antibodies - genetics; Broadly Neutralizing Antibodies - immunology; Broadly Neutralizing Antibodies - metabolism; Cell Surface Display Techniques; Coronaviridae; Coronaviruses; COVID-19; COVID-19 - prevention & control; COVID-19 - therapy; COVID-19 Serotherapy; Directed evolution; Directed Molecular Evolution; Domains; Epitopes; Epitopes - immunology; Human populations; Humans; Immunization, Passive; Immunoglobulin Fc Fragments - immunology; Immunology; Lungs; Mice; Mice, Inbred BALB C; Microbio; Monoclonal antibodies; Neutralization; Pandemics; Progeny; Protein Domains; Protein Engineering; Receptors; Receptors, Coronavirus - metabolism; Respiratory diseases; SARS-CoV-2 - immunology; Severe Acute Respiratory Syndrome - immunology; Severe Acute Respiratory Syndrome - prevention & control; Severe Acute Respiratory Syndrome - therapy; Severe acute respiratory syndrome coronavirus 2; Severe acute respiratory syndrome-related coronavirus - immunology; Spike Glycoprotein, Coronavirus - immunology; Spike Glycoprotein, Coronavirus - metabolism; Viral diseases; Viruses; Yeast

As we continue to battle the COVID-19 pandemic, we must confront the possibility of new pathogenic coronaviruses emerging in humans in the future. With this in mind, Rappazzo et al. isolated antibodies from a survivor of the 2003 severe acute respiratory syndrome coronavirus (SARS-CoV), used yeast d...