Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

• Published in: RSC advances Vol. 1; no. 56; pp. 3488 - 3498
• Main Authors: Ji, Minghui, Xia, Yun, Loo, Jacky Fong-Chuen, Li, Lang, Ho, Ho-Pui, He, Jianan, Gu, Dayong
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 15.09.2020; The Royal Society of Chemistry
• Subjects: accuracy; Assaying; assays; Automation; Chemistry; Coronavirus infections; Coronaviruses; COVID-19; detection; Diagnosis; DNA polymerase; DNA-directed DNA polymerase; early diagnosis; Health services; Infections; Influenza; lead; Magnesium chloride; Microfluidics; mixed infection; Multiplexing; Orthomyxoviridae; pandemic; patients; pharynx; point-of-care systems; Public health; quantitative polymerase chain reaction; rapid methods; Respiratory diseases; reverse transcription; RNA; sampling; screening; Severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus 2; Signs and symptoms; testing; Viral diseases; Viral infections; Viruses

The coronavirus disease 2019 (COVID-19) pandemic, caused by the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, has posed a threat to public health worldwide. Also, influenza virus has caused a large number of deaths annually. Since co-infection of SARS-CoV-2 and influenza vi...