New structural insights into the role of TROVE2 complexes in the on-set and pathogenesis of systemic lupus erythematosus determined by a combination of QCM-D and DPI

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 19; pp. 4709 - 4720
• Main Authors: Juste-Dolz, Augusto, do Nascimento, Noelle M., Monzó, Isidro, Grau-García, Elena, Román-Ivorra, Jose A., Lopez-Paz, José Luis, Escorihuela, Jorge, Puchades, Rosa, Morais, Sergi, Gimenez-Romero, David, Maquieira, Ángel
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2019; Springer; Springer Nature B.V
• Subjects: Analysis; Analytical Chemistry; antigen-antibody complex; Antigenic determinants; Autoantibodies; Autoantibodies - immunology; Autoantigens - chemistry; Autoantigens - immunology; Autoantigens - physiology; Autoimmune diseases; Autoimmunity; Belimumab; Biochemistry; Biomarkers; Calcium; Case-Control Studies; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chronic conditions; Complementarity; Data processing; Epitope mapping; epitopes; Ethylenediaminetetraacetic acid; Fc receptors; Female; Food Science; Humans; Immunotherapy; in vitro studies; Interferometry; Interferometry - methods; Intracellular signalling; Laboratory Medicine; Lupus; lupus erythematosus; Lupus Erythematosus, Systemic - immunology; Male; Medical research; Medicine, Experimental; Microbalances; Monitoring/Environmental Analysis; Pathogenesis; patients; Peptide mapping; Phosphoproteins; Protein Conformation; Protein structure; protein tertiary structure; Proteins; Quartz crystal microbalance; Quartz Crystal Microbalance Techniques; Quartz crystals; Receptors; Research Paper; Ribonucleoproteins - chemistry; Ribonucleoproteins - immunology; Ribonucleoproteins - physiology; RNA, Small Cytoplasmic - chemistry; RNA, Small Cytoplasmic - immunology; RNA, Small Cytoplasmic - physiology; Self-recognition; Systemic lupus erythematosus; Tertiary structure; Young Investigators in (Bio-)Analytical Chemistry; Spain; Dual polarization interferometry; Epitope mapping; Antibody bipolar bridging; Systemic lupus erythematosus; TROVE2; QCM-D

The mechanism of self-recognition of the autoantigen TROVE2, a common biomarker in autoimmune diseases, has been studied with a quartz crystal microbalance with dissipation monitoring (QCM-D) and dual polarization interferometry (DPI). The complementarity and remarkable analytical features of both t...