DNA vaccine protection against SARS-CoV-2 in rhesus macaques

• Published in: Science (American Association for the Advancement of Science) Vol. 369; no. 6505; pp. 806 - 811
• Main Authors: Yu, Jingyou, Tostanoski, Lisa H., Peter, Lauren, Mercado, Noe B., McMahan, Katherine, Mahrokhian, Shant H., Nkolola, Joseph P., Liu, Jinyan, Li, Zhenfeng, Chandrashekar, Abishek, Martinez, David R., Loos, Carolin, Atyeo, Caroline, Fischinger, Stephanie, Burke, John S., Slein, Matthew D., Chen, Yuezhou, Zuiani, Adam, Lelis, Felipe J. N., Travers, Meghan, Habibi, Shaghayegh, Pessaint, Laurent, Van Ry, Alex, Blade, Kelvin, Brown, Renita, Cook, Anthony, Finneyfrock, Brad, Dodson, Alan, Teow, Elyse, Velasco, Jason, Zahn, Roland, Wegmann, Frank, Bondzie, Esther A., Dagotto, Gabriel, Gebre, Makda S., He, Xuan, Jacob-Dolan, Catherine, Kirilova, Marinela, Kordana, Nicole, Lin, Zijin, Maxfield, Lori F., Nampanya, Felix, Nityanandam, Ramya, Ventura, John D., Wan, Huahua, Cai, Yongfei, Chen, Bing, Schmidt, Aaron G., Wesemann, Duane R., Baric, Ralph S., Alter, Galit, Andersen, Hanne, Lewis, Mark G., Barouch, Dan H.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 14.08.2020; American Association for the Advancement of Science
• Subjects: Adjuvants, Immunologic; Alveoli; Animals; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - immunology; Betacoronavirus - immunology; Betacoronavirus - physiology; Bronchoalveolar Lavage Fluid - virology; Bronchus; Coronaviridae; Coronavirus Infections - immunology; Coronavirus Infections - prevention & control; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 Vaccines; Deoxyribonucleic acid; Disease Models, Animal; DNA; DNA vaccines; Female; Humans; Immune response (cell-mediated); Immune response (humoral); Immunity, Cellular; Immunity, Humoral; Immunization, Secondary; Immunogenicity, Vaccine; Immunologic Memory; Immunology; Macaca mulatta; Male; Microbio; Mucosa; Mutant Proteins - chemistry; Mutant Proteins - immunology; Nasal Mucosa - virology; Neutralizing; Pandemics; Pandemics - prevention & control; Pneumonia, Viral - immunology; Pneumonia, Viral - prevention & control; Pneumonia, Viral - virology; Primates; Protein Domains; Proteins; Prototypes; Respiratory diseases; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Spike protein; Vaccination; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; Viral diseases; Viral Load; Viral Vaccines - administration & dosage; Viral Vaccines - immunology; Viruses

The development of a vaccine to protect against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an urgent biomedical need. Yu et al. designed a series of prototype DNA vaccines against the SARS-CoV-2 spike protein, which is used by the virus to bind and invade human cells. Analysis o...