Characterization of transgenic Trifolium subterraneum L. which expresses phyA and releases extracellular phytase: growth and P nutrition in laboratory media and soil

• Published in: Plant, cell and environment Vol. 27; no. 11; pp. 1351 - 1361
• Main Authors: GEORGE, T. S., RICHARDSON, A. E., HADOBAS, P. A., SIMPSON, R. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.11.2004; Blackwell; Wiley Subscription Services, Inc
• Subjects: Aspergillus niger; Biological and medical sciences; Enzymes; Fundamental and applied biological sciences. Psychology; Metabolism; organic P; phosphatase; phytate; Plant physiology and development; rhizosphere; subterranean clover; Trifolium subterraneum; organic P; Growth; 6-Phytase; Phosphoric monoester hydrolases; Esterases; Biomass; Trifolium subterraneum; phytate; Fungi; Gene; Dicotyledones; Angiospermae; Fungi Imperfecti; Thallophyta; Root; Enzyme; Rhizosphere; Leguminosae; Aspergillus niger; Pasture; subterranean clover; Hydrolases; Above ground plant part; Spermatophyta; Exudation; phosphatase

ABSTRACT Transgenic Trifolium subterraneum expressing a phytase gene (phyA) from Aspergillus niger were generated. Five independently transformed lines showed an average 77‐fold increase in exuded phytase activity in comparison with null segregant and wild‐type controls. Unlike other phosphatases, e...