Preparation of crystals for characterizing the Grb7 SH2 domain before and after complex formation with a bicyclic peptide antagonist

• Published in: Acta crystallographica. Section F, Structural biology communications Vol. 70; no. 2; pp. 182 - 186
• Main Authors: Ambaye, Nigus D., Gunzburg, Menachem J., Traore, Daouda A. K., Del Borgo, Mark P., Perlmutter, Patrick, Wilce, Matthew C. J., Wilce, Jacqueline A.
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.02.2014; Wiley Subscription Services, Inc
• Subjects: Amino Acid Sequence; Crystallization; Crystallization Communications; Crystallography, X-Ray; Crystals; Data collection; Grb7; GRB7 Adaptor Protein - chemistry; Humans; Peptides, Cyclic - chemistry; Protein Conformation; seeding; SH2 domain; src Homology Domains; Grb7; seeding; SH2 domain
• ISSN: 2053-230X; 2053-230X
• DOI: 10.1107/S2053230X13033414

Human growth factor receptor‐bound protein 7 (Grb7) is an adapter protein involved in cell growth, migration and proliferation. It is now recognized that Grb7 is an emerging therapeutic target in specific cancer subtypes. Recently, the discovery of a bicyclic peptide inhibitor that targets the Grb7...