Overexpression of pregnane X and glucocorticoid receptors and the regulation of cytochrome P450 in human epileptic brain endothelial cells

• Published in: Epilepsia (Copenhagen) Vol. 58; no. 4; pp. 576 - 585
• Main Authors: Ghosh, Chaitali, Hossain, Mohammed, Solanki, Jesal, Najm, Imad M., Marchi, Nicola, Janigro, Damir
• Format: Journal Article
• Language: English
• Published: United States Wiley 01.04.2017; John Wiley and Sons Inc
• Subjects: Analysis of Variance; Brain - pathology; Cells, Cultured; Cytochrome P-450 Enzyme System - metabolism; Drug resistance; Drug Resistant Epilepsy - pathology; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Epilepsy; Full‐Length Original Research; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Enzymologic - physiology; Glial Fibrillary Acidic Protein - metabolism; Humans; Life Sciences; Neurovascular unit; Nuclear Receptors; Oligonucleotide Array Sequence Analysis; Phosphopyruvate Hydratase - metabolism; Proliferating Cell Nuclear Antigen - metabolism; Receptors, Glucocorticoid - genetics; Receptors, Glucocorticoid - metabolism; Receptors, Steroid - genetics; Receptors, Steroid - metabolism; RNA, Messenger - metabolism; RNA, Small Interfering - pharmacology; Subcellular Fractions - metabolism; Neurovascular unit; Drug resistance; Epilepsy; Nuclear Receptors

Summary Objective Recent evidence suggests a metabolic contribution of cytochrome P450 enzymes (CYPs) to the drug‐resistant phenotype in human epilepsy. However, the upstream molecular regulators of CYP in the epileptic brain remain understudied. We therefore investigated the expression and function...