Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants

• Published in: Plant biotechnology journal Vol. 17; no. 5; pp. 858 - 868
• Main Authors: Li, Jianying, Manghwar, Hakim, Sun, Lin, Wang, Pengcheng, Wang, Guanying, Sheng, Hanyan, Zhang, Jie, Liu, Hao, Qin, Lei, Rui, Hangping, Li, Bo, Lindsey, Keith, Daniell, Henry, Jin, Shuangxia, Zhang, Xianlong
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2019; John Wiley and Sons Inc
• Subjects: biotechnology; Cell division; Comparative analysis; Cotton; CRISPR; CRISPR-Associated Protein 9; CRISPR-Cas Systems; CRISPR/Cas9; Crop improvement; DNA sequencing; Gene Editing; Gene sequencing; Genes; Genetic aspects; Genetic diversity; Genetic Variation - genetics; Genome, Plant - genetics; Genome-Wide Association Study; Genomes; Genomics; Gossypium - genetics; Mutation; Mutation - genetics; Nucleotide sequencing; Nucleotides; off‐target; Plants; Plants, Genetically Modified - genetics; pre‐existing/inherent variation; Proteins; R&D; Research & development; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Software; Somaclonal variation; Target recognition; Transcription factors; Whole Genome Sequencing; whole genome sequencing; cotton; pre-existing/inherent variation; somaclonal variation; CRISPR/Cas9; off-target

Summary The CRISPR/Cas9 system has been extensively applied for crop improvement. However, our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on‐ and off‐target mutation in an edited crop, we described whole genome sequencing (WGS) of 14 Cas9‐edited cotton plant...