Molecular detection of tick-borne protozoan parasites in sika deer (Cervus nippon) from western regions of Japan

• Published in: Parasitology international Vol. 79; p. 102161
• Main Authors: Masatani, Tatsunori, Hayashi, Kei, Morikawa, Momoko, Ozawa, Makoto, Kojima, Isshu, Okajima, Misuzu, Takano, Ai, Shimoda, Hiroshi, Maeda, Ken, Matsuu, Aya, Yoshida, Ayako
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2020
• Subjects: Animals; Apicomplexa - isolation & purification; Babesia; blood; Blood - parasitology; Cervus nippon; Deer; detection; DNA; genes; Hepatozoon; infection; Japan; Japan - epidemiology; liver; Liver - parasitology; parasitology; PCR; polymerase chain reaction; Polymerase Chain Reaction - veterinary; Prevalence; Protozoa; Protozoan Infections, Animal - epidemiology; Protozoan Infections, Animal - parasitology; RNA, Protozoan - analysis; RNA, Ribosomal, 18S - analysis; sampling; screening; sequence analysis; Siberia; Sika deer (Cervus nippon); Theileria; Tick-Borne Diseases - blood; Tick-Borne Diseases - epidemiology; Tick-Borne Diseases - veterinary; ticks; wildlife; Japan; Theileria; Japan; PCR; Sika deer (Cervus nippon); Babesia

The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicom...