Molecular detection of tick-borne protozoan parasites in sika deer (Cervus nippon) from western regions of Japan

The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicom...

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Published inParasitology international Vol. 79; p. 102161
Main Authors Masatani, Tatsunori, Hayashi, Kei, Morikawa, Momoko, Ozawa, Makoto, Kojima, Isshu, Okajima, Misuzu, Takano, Ai, Shimoda, Hiroshi, Maeda, Ken, Matsuu, Aya, Yoshida, Ayako
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.12.2020
Subjects
Animals
Apicomplexa - isolation & purification
Babesia
blood
Blood - parasitology
Cervus nippon
Deer
detection
DNA
genes
Hepatozoon
infection
Japan
Japan - epidemiology
liver
Liver - parasitology
parasitology
PCR
polymerase chain reaction
Polymerase Chain Reaction - veterinary
Prevalence
Protozoa
Protozoan Infections, Animal - epidemiology
Protozoan Infections, Animal - parasitology
RNA, Protozoan - analysis
RNA, Ribosomal, 18S - analysis
sampling
screening
sequence analysis
Siberia
Sika deer (Cervus nippon)
Theileria
Tick-Borne Diseases - blood
Tick-Borne Diseases - epidemiology
Tick-Borne Diseases - veterinary
ticks
wildlife
Japan
Theileria
Japan
PCR
Sika deer (Cervus nippon)
Babesia
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Abstract The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicomplexan parasites (Babesia, Theileria, and Hepatozoon spp.) from 276 blood and liver samples from sika deer captured in the Yamaguchi, Oita, Kagoshima, Okayama, Ehime, Kochi, and Tokushima Prefectures. In total, 259 samples (259/276; 93.8%) tested positive in the nested PCR screening. Gene sequencing revealed that 99.6% (258/259) of positive samples contained Theileria sp. (sika 1), while Theileria sp. (sika 2), another Theileria species, was detected in only 3 samples. We also found that one sample from a sika deer captured in Kagoshima contained the gene of an unidentified Babesia sp. related to Babesia sp. Kh-Hj42, which was previously collected from tick in western Siberia. In conclusion, we found a high prevalence of piroplasms in sika deer from western Japan, and DNA analysis revealed that Theileria sp. (sika 1) had the highest infection rate.
AbstractList The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicomplexan parasites (Babesia, Theileria, and Hepatozoon spp.) from 276 blood and liver samples from sika deer captured in the Yamaguchi, Oita, Kagoshima, Okayama, Ehime, Kochi, and Tokushima Prefectures. In total, 259 samples (259/276; 93.8%) tested positive in the nested PCR screening. Gene sequencing revealed that 99.6% (258/259) of positive samples contained Theileria sp. (sika 1), while Theileria sp. (sika 2), another Theileria species, was detected in only 3 samples. We also found that one sample from a sika deer captured in Kagoshima contained the gene of an unidentified Babesia sp. related to Babesia sp. Kh-Hj42, which was previously collected from tick in western Siberia. In conclusion, we found a high prevalence of piroplasms in sika deer from western Japan, and DNA analysis revealed that Theileria sp. (sika 1) had the highest infection rate.The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicomplexan parasites (Babesia, Theileria, and Hepatozoon spp.) from 276 blood and liver samples from sika deer captured in the Yamaguchi, Oita, Kagoshima, Okayama, Ehime, Kochi, and Tokushima Prefectures. In total, 259 samples (259/276; 93.8%) tested positive in the nested PCR screening. Gene sequencing revealed that 99.6% (258/259) of positive samples contained Theileria sp. (sika 1), while Theileria sp. (sika 2), another Theileria species, was detected in only 3 samples. We also found that one sample from a sika deer captured in Kagoshima contained the gene of an unidentified Babesia sp. related to Babesia sp. Kh-Hj42, which was previously collected from tick in western Siberia. In conclusion, we found a high prevalence of piroplasms in sika deer from western Japan, and DNA analysis revealed that Theileria sp. (sika 1) had the highest infection rate.
The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan parasites in wild sika deer living in western Japan. We used nested polymerase chain reaction (PCR) to detect the 18S rRNA gene of tick-borne apicomplexan parasites (Babesia, Theileria, and Hepatozoon spp.) from 276 blood and liver samples from sika deer captured in the Yamaguchi, Oita, Kagoshima, Okayama, Ehime, Kochi, and Tokushima Prefectures. In total, 259 samples (259/276; 93.8%) tested positive in the nested PCR screening. Gene sequencing revealed that 99.6% (258/259) of positive samples contained Theileria sp. (sika 1), while Theileria sp. (sika 2), another Theileria species, was detected in only 3 samples. We also found that one sample from a sika deer captured in Kagoshima contained the gene of an unidentified Babesia sp. related to Babesia sp. Kh-Hj42, which was previously collected from tick in western Siberia. In conclusion, we found a high prevalence of piroplasms in sika deer from western Japan, and DNA analysis revealed that Theileria sp. (sika 1) had the highest infection rate.
ArticleNumber 102161
Author Matsuu, Aya
Hayashi, Kei
Masatani, Tatsunori
Kojima, Isshu
Takano, Ai
Morikawa, Momoko
Yoshida, Ayako
Ozawa, Makoto
Okajima, Misuzu
Maeda, Ken
Shimoda, Hiroshi
Author_xml – sequence: 1
  givenname: Tatsunori
  surname: Masatani
  fullname: Masatani, Tatsunori
  email: tatsunorimasatani@gmail.com, masatani@vet.kagoshima-u.ac.jp
  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
– sequence: 2
  givenname: Kei
  surname: Hayashi
  fullname: Hayashi, Kei
  organization: Laboratory of Parasitology, Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari 794-8555, Ehime, Japan
– sequence: 3
  givenname: Momoko
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  fullname: Morikawa, Momoko
  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
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  givenname: Makoto
  surname: Ozawa
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  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
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  givenname: Isshu
  surname: Kojima
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  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
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  givenname: Misuzu
  surname: Okajima
  fullname: Okajima, Misuzu
  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
– sequence: 7
  givenname: Ai
  surname: Takano
  fullname: Takano, Ai
  organization: Laboratory of Veterinary Epidemiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
– sequence: 8
  givenname: Hiroshi
  surname: Shimoda
  fullname: Shimoda, Hiroshi
  organization: Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
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  fullname: Maeda, Ken
  organization: Joint Graduate School of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
– sequence: 10
  givenname: Aya
  surname: Matsuu
  fullname: Matsuu, Aya
  organization: Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan
– sequence: 11
  givenname: Ayako
  surname: Yoshida
  fullname: Yoshida, Ayako
  organization: Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan
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Keywords Theileria
Japan
PCR
Sika deer (Cervus nippon)
Babesia
Language English
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Snippet The sika deer (Cervus nippon) is one of the most common species of wildlife in Japan. This study aimed to reveal the prevalence of tick-borne protozoan...
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SubjectTerms Animals
Apicomplexa - isolation & purification
Babesia
blood
Blood - parasitology
Cervus nippon
Deer
detection
DNA
genes
Hepatozoon
infection
Japan
Japan - epidemiology
liver
Liver - parasitology
parasitology
PCR
polymerase chain reaction
Polymerase Chain Reaction - veterinary
Prevalence
Protozoa
Protozoan Infections, Animal - epidemiology
Protozoan Infections, Animal - parasitology
RNA, Protozoan - analysis
RNA, Ribosomal, 18S - analysis
sampling
screening
sequence analysis
Siberia
Sika deer (Cervus nippon)
Theileria
Tick-Borne Diseases - blood
Tick-Borne Diseases - epidemiology
Tick-Borne Diseases - veterinary
ticks
wildlife
Title Molecular detection of tick-borne protozoan parasites in sika deer (Cervus nippon) from western regions of Japan
URI https://www.clinicalkey.com/#!/content/1-s2.0-S1383576920301112
https://dx.doi.org/10.1016/j.parint.2020.102161
https://www.ncbi.nlm.nih.gov/pubmed/32569748
https://www.proquest.com/docview/2416275388
https://www.proquest.com/docview/2498287343
Volume 79
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