Single Amino Acid Substitution in the Matrix Protein of Rabies Virus Is Associated with Neurovirulence in Mice

• Published in: Viruses Vol. 16; no. 5; p. 699
• Main Authors: Harada, Michiko, Matsuu, Aya, Kaku, Yoshihiro, Okutani, Akiko, Inoue, Yusuke, Posadas-Herrera, Guillermo, Inoue, Satoshi, Maeda, Ken
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2024
• Subjects: Adaptation; adults; Amino Acid Substitution; Analysis; Animals; Bats; Body weight; body weight changes; Brain; Brain - pathology; Brain - virology; Care and treatment; Cell Line; Cell lines; chickens; Cloning; Diagnosis; Embryo fibroblasts; Fibroblasts; Genomes; Health aspects; Infections; Infectious diseases; Inoculation; Laboratory animals; M protein; Matrix protein; Mice; mouse neuroblastoma cell; Nervous system; Neuroblastoma; neurons; Neurons - pathology; Neurons - virology; Neurovirulence; Plasmids; Propagation; Proteins; Rabies; Rabies - virology; Rabies lyssavirus; rabies virus; Rabies virus - genetics; Rabies virus - pathogenicity; RNA polymerase; RNA polymerases; Scientific equipment and supplies industry; Variance analysis; viral growth; Viral Matrix Proteins - genetics; Viral Matrix Proteins - metabolism; Virulence; Virulence (Microbiology); Virus Replication; Viruses; United States; Japan; United States > US; Germany; mouse neuroblastoma cell; rabies virus; amino acid substitution; matrix protein

Rabies is a fatal encephalitic infectious disease caused by the rabies virus (RABV). RABV is highly neurotropic and replicates in neuronal cell lines in vitro. The RABV fixed strain, HEP-Flury, was produced via passaging in primary chicken embryonic fibroblast cells. HEP-Flury showed rapid adaptatio...