Regulation of taurocholate excretion by a hypo-osmolarity-activated signal transduction pathway in rat liver
Hypo-osmotic cell swelling increases the capacity of taurocholate excretion into bile in the perfused rat liver. The aim of this study was to clarify the mechanisms linking cell swelling to bile acid secretion. The influence of hypo-osmotic cell swelling on intracellular signal transduction and bile...
Saved in:
Published in | Gastroenterology (New York, N.Y. 1943) Vol. 110; no. 3; p. 858 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.1996
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Hypo-osmotic cell swelling increases the capacity of taurocholate excretion into bile in the perfused rat liver. The aim of this study was to clarify the mechanisms linking cell swelling to bile acid secretion.
The influence of hypo-osmotic cell swelling on intracellular signal transduction and bile acid secretion was studied in isolated rat hepatocytes and the perfused rat liver.
In rat livers perfused with hypo-osmotic buffer (225 mOsm/L), the maximum velocity of taurocholate excretion into bile is increased by 135% within 10-20 minutes. To unravel signaling events mediating this effect, the activities of the mitogen-activated protein kinases, extracellular-signal-regulated kinase (Erk)-1 and Erk-2, were measured after hypo-osmotic treatment in cultured rat hepatocytes. A rapid parallel activation of Erk-1 and Erk-2 was observed within 1 minute, which became maximal after 10 minutes and returned to the basal level within 60 minutes. The hypo-osmolarity-induced Erk activation and the increase in bile flow after hypo-osmotic liver perfusion were completely abolished by inhibitors of signal transduction at the level of G proteins and tyrosine kinases but remained unaffected by the inhibition of the protein kinase C.
A G protein-and tyrosine kinase-dependent but protein kinase C-independent activation of mitogen-activated protein kinases is involved in the regulation of taurocholate excretion by liver cell hydration changes. |
---|---|
AbstractList | Hypo-osmotic cell swelling increases the capacity of taurocholate excretion into bile in the perfused rat liver. The aim of this study was to clarify the mechanisms linking cell swelling to bile acid secretion.
The influence of hypo-osmotic cell swelling on intracellular signal transduction and bile acid secretion was studied in isolated rat hepatocytes and the perfused rat liver.
In rat livers perfused with hypo-osmotic buffer (225 mOsm/L), the maximum velocity of taurocholate excretion into bile is increased by 135% within 10-20 minutes. To unravel signaling events mediating this effect, the activities of the mitogen-activated protein kinases, extracellular-signal-regulated kinase (Erk)-1 and Erk-2, were measured after hypo-osmotic treatment in cultured rat hepatocytes. A rapid parallel activation of Erk-1 and Erk-2 was observed within 1 minute, which became maximal after 10 minutes and returned to the basal level within 60 minutes. The hypo-osmolarity-induced Erk activation and the increase in bile flow after hypo-osmotic liver perfusion were completely abolished by inhibitors of signal transduction at the level of G proteins and tyrosine kinases but remained unaffected by the inhibition of the protein kinase C.
A G protein-and tyrosine kinase-dependent but protein kinase C-independent activation of mitogen-activated protein kinases is involved in the regulation of taurocholate excretion by liver cell hydration changes. |
Author | Häussinger, D Schliess, F Heinrich, S Noé, B Wettstein, M |
Author_xml | – sequence: 1 givenname: B surname: Noé fullname: Noé, B organization: Clinic for Gastroenterology, Hepatology, and Infectiology, Heinrich-Heine-Universität, Düssseldorf, Germany – sequence: 2 givenname: F surname: Schliess fullname: Schliess, F – sequence: 3 givenname: M surname: Wettstein fullname: Wettstein, M – sequence: 4 givenname: S surname: Heinrich fullname: Heinrich, S – sequence: 5 givenname: D surname: Häussinger fullname: Häussinger, D |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8608896$$D View this record in MEDLINE/PubMed |
BookMark | eNotj91qgzAcxXPR0bXdHmGQF4hL1KTxcpR9QWEwel_-JlEz1EgS3Xz7SdurA-f8zoGzRave9QYhzGjCKM-eawgxYUUhkokt3tBJQaUsxAptKGWCcCr5PdqG8EMpLTLJ1mh9Qzao_Tb12EK0rseuwhFG71TjFsdg86e8uSTljAE38-CIC90SehtnAiraaeE0DrbuocXRQx_0qC6VAWLzCzO2PfYQcWsn4x_QXQVtMI833aHT2-vp8EGOX--fh5cjUZzlkWSK6ZILbaCUOU8rKiQDUVXc5ACsLIwGlvNsD4ouzF6INNeqYFQrraqSpzv0dJ0dxrIz-jx424Gfz7fT6T_kKmBX |
CitedBy_id | crossref_primary_10_1016_j_exer_2007_02_007 crossref_primary_10_1096_fasebj_13_12_1557 crossref_primary_10_4238_2012_October_9_2 crossref_primary_10_1006_abbi_1997_0517 crossref_primary_10_1002_hep_510290227 crossref_primary_10_1006_bbrc_1998_9946 crossref_primary_10_1006_jsre_2000_5866 crossref_primary_10_1515_hsz_2021_0193 crossref_primary_10_1016_j_mam_2013_05_001 crossref_primary_10_1074_jbc_M111_292896 crossref_primary_10_1074_jbc_M205945200 crossref_primary_10_1523_JNEUROSCI_0120_06_2006 crossref_primary_10_1097_00006676_200008000_00013 crossref_primary_10_1523_JNEUROSCI_18_04_01196_1998 crossref_primary_10_1016_S0168_8278_98_80229_8 crossref_primary_10_1074_jbc_273_36_22962 crossref_primary_10_1053_j_gastro_2003_09_044 crossref_primary_10_1515_BC_2000_063 crossref_primary_10_1538_expanim_49_9 crossref_primary_10_1016_S0014_5793_96_01376_2 crossref_primary_10_1586_eem_12_75 crossref_primary_10_1152_ajpgi_1999_277_6_G1165 crossref_primary_10_1002_jcb_1205 crossref_primary_10_1016_S0014_5793_99_01719_6 crossref_primary_10_1152_ajpgi_1998_274_3_G544 crossref_primary_10_1002_ar_a_20094 crossref_primary_10_1016_j_bbagen_2016_05_032 crossref_primary_10_3748_wjg_14_5620 crossref_primary_10_1046_j_1472_8206_2003_00197_x crossref_primary_10_1097_00054725_199706000_00005 crossref_primary_10_1002_hep_25955 crossref_primary_10_1016_j_jhep_2004_04_035 crossref_primary_10_1002_hep_510280326 crossref_primary_10_1053_gast_2001_26262 crossref_primary_10_1016_j_lfs_2007_07_008 crossref_primary_10_1006_jsre_2001_6225 crossref_primary_10_1515_BC_1999_162 crossref_primary_10_1016_S0016_5085_03_01055_2 crossref_primary_10_1016_S0014_2999_00_00076_5 crossref_primary_10_1016_S0016_5085_03_00274_9 crossref_primary_10_1080_10408398_2018_1481822 crossref_primary_10_3748_wjg_14_6786 crossref_primary_10_1515_BC_2001_133 crossref_primary_10_1016_j_jhep_2005_01_001 crossref_primary_10_1089_ars_2021_0021 crossref_primary_10_1002_cbf_2916 crossref_primary_10_1152_physrev_1998_78_1_247 crossref_primary_10_1159_000335845 crossref_primary_10_1016_S0014_5793_99_00643_2 crossref_primary_10_1074_jbc_M210699200 crossref_primary_10_1016_S1091_255X_01_80094_9 crossref_primary_10_1016_S0016_5085_99_70138_1 crossref_primary_10_1053_gast_1999_0029900433 crossref_primary_10_2174_0929867325666171205153204 crossref_primary_10_1093_jn_131_9_2509S crossref_primary_10_1074_jbc_M002831200 crossref_primary_10_1074_jbc_M307603200 crossref_primary_10_1074_jbc_M115_666883 crossref_primary_10_1111_j_1469_7793_2001_00347_x crossref_primary_10_1152_ajpgi_00355_2003 crossref_primary_10_1053_j_gastro_2003_11_003 crossref_primary_10_1007_s11011_007_9062_5 crossref_primary_10_1074_jbc_273_41_26638 crossref_primary_10_1016_S0014_5793_98_01030_8 crossref_primary_10_1002_hep_510280423 crossref_primary_10_1002_hep_20090 crossref_primary_10_1016_S0168_8278_00_80411_0 crossref_primary_10_1074_jbc_273_24_14906 crossref_primary_10_1111_j_1748_1716_2006_01542_x crossref_primary_10_1007_BF01149599 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1053/gast.1996.v110.pm8608896 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine |
ExternalDocumentID | 8608896 |
Genre | Journal Article |
GroupedDBID | --- --K .1- .55 .FO .GJ 0R~ 1B1 1CY 1P~ 1~5 3O- 4.4 457 4G. 53G 5GY 5RE 5VS 7-5 AAEDT AAEDW AAIKJ AALRI AAQFI AAQOH AAQQT AAQXK AAXUO AAYOK ABCQX ABJNI ABLJU ABMAC ABOCM ADBBV ADMUD ADPAM AENEX AEVXI AFCTW AFFNX AFHKK AFJKZ AFRHN AFTJW AGHFR AI. AITUG AJUYK ALMA_UNASSIGNED_HOLDINGS AMRAJ ASPBG AVWKF AZFZN BELOY BR6 C5W CAG CGR COF CS3 CUY CVF DU5 EBS ECM EFJIC EIF EJD F5P FD8 FDB FEDTE FGOYB G8K GBLVA HVGLF HZ~ IHE J1W J5H K-O KOM L7B M41 MO0 N4W N9A NPM NQ- O9- OC. OHT ON0 P2P PC. QTD R2- RIG ROL RPZ SEL SES SJN SSZ TWZ UDS UGJ UV1 VH1 WH7 X7M XH2 Y6R YQJ Z5R ZGI ZXP |
ID | FETCH-LOGICAL-c514t-3c1db56deab8452f0681a6ff5e4aa1b9eda14537ac0dea76624dc910dcdcfb52 |
ISSN | 0016-5085 |
IngestDate | Sat Sep 28 08:39:32 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c514t-3c1db56deab8452f0681a6ff5e4aa1b9eda14537ac0dea76624dc910dcdcfb52 |
OpenAccessLink | http://www.gastrojournal.org/article/S001650859600131X/pdf |
PMID | 8608896 |
ParticipantIDs | pubmed_primary_8608896 |
PublicationCentury | 1900 |
PublicationDate | 1996-03-01 |
PublicationDateYYYYMMDD | 1996-03-01 |
PublicationDate_xml | – month: 03 year: 1996 text: 1996-03-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Gastroenterology (New York, N.Y. 1943) |
PublicationTitleAlternate | Gastroenterology |
PublicationYear | 1996 |
SSID | ssj0009381 |
Score | 1.8915919 |
Snippet | Hypo-osmotic cell swelling increases the capacity of taurocholate excretion into bile in the perfused rat liver. The aim of this study was to clarify the... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 858 |
SubjectTerms | Animals Bile - metabolism Calcium-Calmodulin-Dependent Protein Kinases - metabolism Cells, Cultured Enzyme Activation Liver - cytology Liver - enzymology Liver - metabolism Male Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases Osmolar Concentration Protein-Tyrosine Kinases - metabolism Rats Rats, Wistar Signal Transduction Taurocholic Acid - metabolism |
Title | Regulation of taurocholate excretion by a hypo-osmolarity-activated signal transduction pathway in rat liver |
URI | https://www.ncbi.nlm.nih.gov/pubmed/8608896 |
Volume | 110 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9tAEF4FEIgLggLqg1Z74BY5xPbuxj5WCIoqkQMKKje0TxKJ2BExr_6Z_tXOPhKbRyTgYlnexLJ3Po-_WX8zg9A-VZSL3ECkKg0EKIkhkci1inKRSiNzKRPXh-y0z07Oye8LetFq_Wuolm4r0ZF_X80r-YhV4RjY1WbJvsOy85PCAdgH-8IWLAzbN9n4zDeSD5yv4re2_xXEqpVu6wdp8xNhBPglbw8fJ2VUTsc2kAXeHdl0hjtu2aYVcDjBObyzlC8la4utDu-5ywgEgLSvrXajyWJ_8Wl1U9pqnje-hNMrLX3acU6aq7j90n-Td4CqP_8MgQP7vu1zifEfXVXTWRPOxmLtqACfPayXa1VI3mO1WGvmf2MWASWkT_xv0LWOmvG5fxX7qu4vvHzXteq4gnu1yZascwen6EzGGbOaLdb8C9hrMnbWf-vgs-rbYWQJLfUy60D7djFoVtM5zXxDxnBTQSgGV3ew6NrW0WrYexbGODoz2EQbIQ7BPz2otlBLF5_Q2mlQWmyj6xpbuDS4iS08xxYWj5jjhdjCHlu4iS0csIVHBQZsYYetHTQ4PhocnkShM0ckgWBXUSpjJShTmouM0MR0WRZzZgzVhPPYPuw8JjTtcdmF3_QYS4iSQEyVVNIImuyi5aIs9GeEgY3HDOZDQZxBUkKESUmaQgxusm6eGPoF7fppupz46iuXYf6-Lhr4htZr6O2hFQMPu_4OzLESP5z9_gPBK28j |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Regulation+of+taurocholate+excretion+by+a+hypo-osmolarity-activated+signal+transduction+pathway+in+rat+liver&rft.jtitle=Gastroenterology+%28New+York%2C+N.Y.+1943%29&rft.au=No%C3%A9%2C+B&rft.au=Schliess%2C+F&rft.au=Wettstein%2C+M&rft.au=Heinrich%2C+S&rft.date=1996-03-01&rft.issn=0016-5085&rft.volume=110&rft.issue=3&rft.spage=858&rft_id=info:doi/10.1053%2Fgast.1996.v110.pm8608896&rft_id=info%3Apmid%2F8608896&rft_id=info%3Apmid%2F8608896&rft.externalDocID=8608896 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0016-5085&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0016-5085&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0016-5085&client=summon |