Enhanced Bio-Ethanol Production from Industrial Potato Waste by Statistical Medium Optimization

• Published in: International journal of molecular sciences Vol. 16; no. 10; pp. 24490 - 24505
• Main Authors: Izmirlioglu, Gulten, Demirci, Ali
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.10.2015; MDPI
• Subjects: bio-ethanol; Box-Behnken design; Ethanol; Ethanol - metabolism; Industrial Waste; Industrial wastes; Optimization techniques; Plackett-Burman; Potatoes; Saccharomyces - metabolism; Saccharomyces cerevisiae; Solanum tuberosum; Statistical analysis; Plackett-Burman; Box-Behnken design; bio-ethanol; Saccharomyces cerevisiae; industrial waste
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms161024490

Industrial wastes are of great interest as a substrate in production of value-added products to reduce cost, while managing the waste economically and environmentally. Bio-ethanol production from industrial wastes has gained attention because of its abundance, availability, and rich carbon and nitrogen content. In this study, industrial potato waste was used as a carbon source and a medium was optimized for ethanol production by using statistical designs. The effect of various medium components on ethanol production was evaluated. Yeast extract, malt extract, and MgSO₄·7H₂O showed significantly positive effects, whereas KH₂PO₄ and CaCl₂·2H₂O had a significantly negative effect (p-value<0.05). Using response surface methodology, a medium consisting of 40.4 g/L (dry basis) industrial waste potato, 50 g/L malt extract, and 4.84 g/L MgSO₄·7H₂O was found optimal and yielded 24.6 g/L ethanol at 30 °C, 150 rpm, and 48 h of fermentation. In conclusion, this study demonstrated that industrial potato waste can be used effectively to enhance bioethanol production.